introduction: acanthamoeba is an opportunistic protist, which is ubiquitously distributed in the environment. infection with acanthamoeba spp. poses threat to human health, such as, acanthamoeba keratitis (ak) that is a vision-threatening infection of the cornea. this study aimed to identify the species of acanthamoeba strains isolated from cornea of keratitis patients by polymerase chain react...

OBJECTIVE To examine the prevalence of and risk factors for cornea guttata in a rural southwestern island of Japan. DESIGN Cross-sectional, population-based study. All residents of Kumejima Island, Japan, located in southwestern Japan (eastern longitude, 126° 48'; northern latitude, 26° 20'), 40 years or older were asked to undergo a comprehensive questionnaire and ocular examination, includi...

Hydroxyproline (Hyp) concentrations (total, free, peptide-bound and protein-bound) in camel eye tissues were determined. Total Hyp concentration was highest in iris, followed by ciliary body, sclera, cornea, lens and retina; the difference between total Hyp concentration of iris and sclera (P < 0.05) and cornea, lens and retina (P < 0.001) was statistically significant. Cornea had the highest c...

PURPOSE To compare the abundance and spatial distribution of serum albumin in the mouse and bovine cornea. METHODS Serum albumin from cornea was separated from transketolase by SDS-PAGE (+/-dithiothreitol [DTT]) and identified by peptide sequencing and immunoblot analyses. The fractional content of serum albumin was determined in water-soluble extracts of cornea by imaging analyses after SDS-...

PURPOSE To isolate tissue-specific gene products that contribute to corneal integrity. METHODS A cDNA library was constructed and differentially hybridized. Cornea-specific clones were purified and further characterized. RESULTS In this study cornea-specific gene products were isolated by differential cDNA hybridization. In addition to known cornea-specific gene products, a transcript was i...