BACKGROUND/AIMS Herpes simplex virus keratitis (HSK) is the most common cause of corneal blindness in the Western world. Delay in the treatment of HSK can lead to a more significant corneal scar and topical steroid treatment in unsuspected active HSK can lead to corneal melting. Current culture techniques for herpes simplex virus (HSV) take several days and commercially available HSV laboratory...

Corneal scarring remains a major cause of blindness world-wide, with limited treatment options, all of which have side-effects. Here, we tested the hypothesis that topical application of Rosiglitazone, a Thiazolidinedione and ligand of peroxisome proliferator activated receptor gamma (PPARγ), can effectively block scar formation in a cat model of corneal damage. Adult cats underwent bilateral e...

Canine amniotic membrane (AM) transplantation in conjunction with a third eyelid flap was used to promote healing of created deep corneal ulcers in 6 normal mongrel dogs. The healing was compared with the healing of created ulcers in the other eye of the same dog that were treated only with the third eyelid flap. A minimum of 60 days interval was allowed between the two treatment procedures in ...

Background: Corneal perforation is a leading cause of ocular morbidity around the world. It may occur due to trauma eye, keratitis, and many other conditions. Any corneal should be treated immediately promptly avoid complications subsequently blindness.Objective: To describe effectiveness using autologous Tenon’s tissue along with bandage contact lens manage perforations less than 3 mm in size....

PURPOSE The mouse has become an important wound healing model with which to study corneal fibrosis, a frequent complication of refractive surgery. The aim of the current study was to quantify changes in stromal ultrastructure and light scatter that characterize fibrosis in mouse corneal debridement wounds. METHODS Epithelial debridement wounds, with and without removal of basement membrane, w...

PURPOSE Subepithelial haze is a frequent complication and is often the cause of regression after photorefractive keratectomy (PRK). The lack of understanding of this undesirable complication following PRK is in part due to the limited availability of suitable tissues for pathological studies. METHODS We examined the expression of various extracellular components in the cornea of a 46-year-old...

PURPOSE To morphologically, biochemically, and physiologically characterize corneal cross-linking with riboflavin and UV-A light (CXL) in a newly established in vivo murine model. METHODS C57BL/6 wild-type mice (N = 67) were treated with various CXL protocols, with modification of the following parameters: total energy (fluence) used, duration of UV-A irradiation, continuous versus pulsed irr...

PURPOSE To assess and compare corneal modifications induced by IntraLase PulsionFS femtosecond laser and mechanical microkeratome Hansatome for laser in situ keratomileusis (LASIK) using the new-generation Heidelberg Retina Tomograph II (HRT II)/Rostock Cornea Module confocal microscope. METHODS In this case-control study, 24 eyes of 12 patients were examined with the HRT II in the follow-up ...