Notes: “-“ indicates not mentioned in article Therapy: HDF: hemodiafiltration; HD: hemodialysis; CAVH: continuous arteriovenovenous hemofiltration Diagnoses: TF: tibial fracture; POM: polymicrobial osteomyelitis; GD: gastric dysplasia; CD: cardiac disease; VPS: ventriculoperitoneal shunt; PD: Parkinson’s disease; P: pneumonia Vancomycin assay: EMIT: enzyme-multiplied immunoassay technique, Sylv...

This highly sensitive method for determining retinol-binding protein in human serum and urine is based on a double-antibody "sandwich"-type enzyme-linked immunosorbent assay. The assayable concentration range is 0.8-48 micrograms/L, the detection limit 0.2 micrograms/L. Within-assay coefficients of variation for 10 determinations at two different concentrations were 5.5 and 5.8%. The correspond...

BACKGROUND Although measurement of whole-blood creatinine at the point of care offers rapid assessment of renal function, agreement of point-of-care (POC) results with central laboratory methods continues to be a concern. We assessed the influence of several potential interferents on POC whole-blood creatinine measurements. METHODS We compared POC creatinine (Nova StatSensor) measurements wit...

We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly simplifying calculations and improving sensitivity and accuracy. Measurement of c...

We describe a kinetic, fluorometric assay for urinary Nacetyl-j3-glucosaminidase (EC 3.2.1.30) by measurement of the release of 4-methylumbelliferone (7-hydroxy-4-methylcoumarin). The method is simple, involving only a single reagent, and is applicable to the assay of other enzymes that hydrolyze similar fluorescent-labeled substrates. The enzyme distribution in human tissues and fluids is desc...

RESEARCH DESIGN AND METHODS — Type 2 diabetic subjects (n 1,716), inclusive of known diabetic subjects (KD subjects) (1,363 of 1,529; response rate 89.1%) and randomly selected newly diagnosed diabetic subjects (NDD subjects) (n 353) were selected from the Chennai Urban Rural Epidemiology Study (CURES). Microalbuminuria was estimated by immunoturbidometric assay and diagnosed if albumin excreti...

BACKGROUND Urine creatinine concentration often is used in ratios such as urine protein:creatinine to compensate for dilution or concentration of spot urine samples. OBJECTIVE The purpose of this study was to compare the accuracy of different techniques of urine creatinine measurement currently available for veterinary practitioners. METHODS In 104 samples of canine urine diluted 1:20 with ...

BACKGROUND Point-of-care testing for creatinine using a fingerprick sample and resultant estimated glomerular filtration rate has potential for screening for chronic kidney disease in community settings. This study assessed the applicability of the Nova StatSensor creatinine analyzer for this purpose. METHODS Fingerprick samples from 100 patients (63 renal, 37 healthy volunteers; range 46-962...

Background Rapid and accurate estimation of the glomerular filtation rate (GFR) is required for many major clinical decisions in patients with chronic nephropathies [1]. Direct GFR measurement is time-consuming and expensive, frequently requires urine collection and isotope use, and is routinely available in only a few medical centers [1]. In clinical practice, GFR is usually estimated from the...

BACKGROUND Urinary neutrophil gelatinase-associated lipocalin (NGAL) is a novel, sensitive and specific biomarker that is rapidly released after kidney injury. It predicts acute kidney injury (AKI) in multiple clinical scenarios. We hypothesized that urinary NGAL can predict AKI after liver transplantation. METHODS Urine was collected in 92 patients undergoing liver transplantation (18 living...