on to a heavy wooden mill which is revolved by the bulls moving in a circle round the mill. The old man was bending over the crushing blocks to remove sugar-cane debris when his long hair got undone and was caught in the mill. The bulls continued to move round in a circle which resulted in his hair being wound into the mill. He was dragged round the mill and fruitlessly tugged to get free. His ...

In this work, we introduce persistent homology for the analysis of cryo-electron microscopy (cryo-EM) density maps. We identify the topological fingerprint or topological signature of noise, which is widespread in cryo-EM data. For low signal-to-noise ratio (SNR) volumetric data, intrinsic topological features of biomolecular structures are indistinguishable from noise. To remove noise, we empl...

Ball mill is vital equipment in industries viz. mineral dressing, ore processing, fertilizers, food and diary, pharmaceuticals and many others. The present work involves a meticulous study of the effect of the various parameters on the performance of a ball mill. The parameters studied in this work are particle size, number of balls, time of grinding, particle density, and speed of the ball mil...

Neuromodulation is well known to provide plasticity in pattern generating circuits, but few details are available concerning modulation of motor pattern coordination. We are using the crustacean stomatogastric nervous system to examine how co-expressed rhythms are modulated to regulate frequency and maintain coordination. The system produces two related motor patterns, the gastric mill rhythm t...

Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive pa...

Independent cryo electron microscopy (cryo-EM) studies of the closely related protein disaggregases ClpB and Hsp104 have resulted in two different models of subunit arrangement in the active hexamer. We compare the EM maps and resulting atomic structure fits, discuss their differences, and relate them to published experimental information in an attempt to discriminate between models. In additio...

Since the advent of cryo-electron microscopy (cryo-EM), the close-to-life and fine structure preservation of biological samples has become a realized procurement, surpassing the fixation quality and avoiding the numerous artifacts inherent to conventional chemical preservation methods. Cryo-EM techniques such as high-pressure freezing followed by freezesubstitution (HPF/FS) have facilitated the...

We developed a method, named GraFix, that considerably improves sample quality for structure determination by singleparticle electron cryomicroscopy (cryo-EM). GraFix uses a glycerol gradient centrifugation step in which the complexes are centrifuged into an increasing concentration of a chemical fixation reagent to prevent aggregation and to stabilize individual macromolecules. The method can ...

BACKGROUND In addition to minimally invasive destruction of tumors, cryo-ablation of tumors to some extent modulated anti-tumor immunity. Cryo-ablated tumors in glioma mice models induced anti-tumor cellular immunologic response which increases the percentage of CD3(+) and CD4(+)T cells in blood as well as natural killer cells. As a crucial role in triggering anti-tumor immunity, dendritic cell...