Cells and tissues undergo complex physical processes during cryopreservation. Understanding the underlying physical phenomena is critical to improve current cryopreservation methods and to develop new techniques. Here, we describe multi-scale approaches for modelling cell and tissue cryopreservation including heat transfer at macroscale level, crystallization, cell volume change and mass transp...

Cryopreservation is beset with the challenge of protocol alignment across a wide range of cell types and process variables. By taking a cross-sectional assessment of previously published cryopreservation data (sample means and standard errors) as preliminary meta-data, a decision tree learning analysis (DTLA) was performed to develop an understanding of target survival using optimized pruning m...

Cryopreservation is beset with the challenge of protocol alignment across a wide range of cell types and process variables. By taking a cross-sectional assessment of previously published cryopreservation data (sample means and standard errors) as preliminary meta-data, a decision tree learning analysis (DTLA) was performed to develop an understanding of target survival and optimized pruning met...

Laboratories around the world have produced tens of thousands of mutant and transgenic zebrafish lines. As with mice, maintaining all of these valuable zebrafish genotypes is expensive, risky, and beyond the capacity of even the largest stock centers. Because reducing oxidative stress has become an important aspect of reducing the variability in mouse sperm cryopreservation, we examined whether...

Supplementation of vitamin E (α-tocopherol) as an antioxidant into extender is useful to maintain sperm quality during the process cryopreservation by protecting sperms from reactive oxygen species (ROS). This study aims evaluate protective impacts supplementation on motility, viability, and reducing abnormality in Sapera goats. The sample semen used present was obtained fresh good-quality ejac...

Embryo cryopreservation represents a pivotal tool for the long-term storage and exchange of valuable genetic resources of livestock and endangered species. The innumerable applications of embryo cryopreservation in human medicine, animal production, as well as in other embryo biotechnologies for research purposes are calling for standardized protocols that can be used in these different fields....

Cryopreservation of embryos and gametes has become an indispensible service for infertility treatment. Vitrification is now a prevalent technique for cryopreservation in assisted reproductive technology (ART) replacing, in many cases, conventional slow cooling methodology. Fertility preservation in female patients has become a very topical issue. Various malignant and nonmalignant diseases have...

The current study aimed to examine the percentage changes of viability sperm and activity enzymes LDH, ALP, GOT / AST, GPT ALT in plasma Lacaune rams, before after cryopreservation. For this purpose, five rams were examined, two ejaculates obtained from each ram. Ejaculates are collected by method artificial vagina, during insemination campaign. All diluted with a 6AG extender frozen Cassou?s s...

Children and young adults, who suffer from cancer, receive gonadotoxic therapy, which destroys their fertile abilities after survival. Ovarian cryopreservation and transplantation provide the promising solution to this problem, where the ovary can be removed before the gonadotoxic therapy and reimplanted after patient's survival, where the ovary is to be cryopreserved during the period of the t...