We studied the diagnostic performance of the IRIDICA PCR/electrospray ionization-mass spectrometry (PCR/ESI-MS) assay applied on bronchoalveolar lavage (BAL) samples, from 51 mechanically ventilated patients with suspected pneumonia, in a prospective study. In 32 patients with X-ray verified pneumonia, PCR/ESI-MS was positive in 66% and BAL culture was positive in 38% (p = 0.045), and either of...

staphylococcus spp., is a common bacteria in cattle mastitis. detection of the low number of bacteria requires a sensitive method. we used multiplex polymerase chain reaction (m-pcr) assay to detect the genes encoding sea, seb, sec toxins, that are specific in s. aureus bacteria. the gene of 23s rrna that is conserved in all staphylococcus spp., also was used. due to the stiffness and low effec...

background and objectives : each year, enteroviruses infect millions of people and cause different diseases. the agents are usually detected using cell culture. rd (rhabdomyosarcoma) and l20b (l cells) are among the recommended cells by the world health organisation (who) for this purpose. even though cell culture is the most common method used in diagnosing enteroviruses in stool specimens, th...

background and aims: influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. a variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. methods: nasal and pharyngeal samples taken from patients were inoculated into madin-darby canine kidney (mock) cells and embryonated chicken eggs (eces). the culture media was as...

contagious agalactia (c.a) is an infectious syndrome of sheep that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. mycoplasma agalactiae (m. agalactiae) is the main cause of the disease in sheep. the aim of this study was isolation and identification of m. agalactiae with culture and polymerase chain reaction (pcr) assay f...

Introduction: Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women. Materials and methods: Hundred pregnant women, at the 35th and 37th weeks of pregnancy, were selected fro...

Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated...

conclusions performing only culture method led to missed false negative carriers. therefore, it is recommended that both the pcr assay and conventional culture method be routinely performed to detect gbs in pregnant women accurately. pcr diagnosis demonstrated a shorter turnaround time when compared with the time consuming culture method. results the prevalence of gbs in the 203 collected sampl...

We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has bee...