Instantly switch recording modes by using the membrane potential as the trigger. Preset a voltage threshold in current-clamp mode, and MultiClamp 700B automatically switches to voltage-clamp when the membrane potential reaches that voltage level. User-specified delay allows you to perform creative new experiments. Suppress Oscillations Sudden changes in membrane or pipette parameters may result...

1. Insect toxin, mammal toxins I and II and crustacean toxin were obtained from the venom of the scorpion Androctonus australis. Their effects on the isolated giant axon of the cockroach Periplaneta americana were investigated by current-clamp and voltage-clamp techniques. 2. In current-clamp conditions, mammal toxins and crustacean toxin (1*3—13 /IM) induced a large prolongation of the falling...

One of the difficulties with PWM switching converters is high switching loss and electromagnetic interference due to switching at non-zero voltage and current, which limits the operating frequency. In order to reduce the converter volume and weight (by increasing the frequency) and reducing switching losses, zero voltage and current switching methods are recommended. In this paper, four main ze...

One of the difficulties with PWM switching converters is high switching loss and electromagnetic interference due to switching at non-zero voltage and current, which limits the operating frequency. In order to reduce the converter volume and weight (by increasing the frequency) and reducing switching losses, zero voltage and current switching methods are recommended. In this paper, four main ze...

The objective of these studies was to define the roles of calcium and sodium in uterine smooth muscle excitation. The double sucrose-gap technique was used for current-clamp and voltage-clamp experiments. It was shown that neither sodium nor calcium alone is capable of supporting excitation in estrogen-dominated uterine smooth muscle. Calcium dependence was explained in part by increased membra...

Mechanisms underlying action potential generation in the newt olfactory receptor cell were investigated by using the whole-cell version of the patch-clamp technique. Isolated olfactory cells had a resting membrane potential of -70 +/- 9 mV. Injection of a depolarizing current step triggered action potentials under current clamp condition. The amplitude of the action potential was reduced by low...

1. Inactivation of delayed outward current was studied by voltage clamp of isolated neurone somata of the molluscs Archidoris and Anisodoris. During prolonged voltage clamp steps in normal artificial sea water delayed outward current rises to a peak and then declines to a non-zero steady-state. During repetitive clamp pulses at repetition rates slower than 2/sec, the amplitude of peak outward c...

The voltage-clamp technique is applicable only to spherical cells. In nonspherical cells, such as neurons, the membrane potential is not clamped distal to the voltage-clamp electrode. This means that the current recorded by the voltage-clamp electrode is the sum of the local current and of axial currents from locations experiencing different membrane potentials. Furthermore, voltage-gated curre...

Dynamic clamp is a collection of closely related techniques that have been employed in cardiac electrophysiology to provide direct answers to numerous research questions regarding basic cellular mechanisms of action potential formation, action potential transfer and action potential synchronization in health and disease. Building on traditional current clamp, dynamic clamp was initially used to...