IFNs, which transduce pivotal signals through Stat1 and Stat2, effectively suppress the replication of Legionella pneumophila in primary murine macrophages. Although the ability of IFN-γ to impede L. pneumophila growth is fully dependent on Stat1, IFN-αβ unexpectedly suppresses L. pneumophila growth in both Stat1- and Stat2-deficient macrophages. New studies demonstrating that the robust respon...

T cell commitment and αβ/γδ lineage specification in the thymus involves interactions between many different genes. Characterization of these interactions thus requires a multiparameter analysis of individual thymocytes. We developed two efficient single-cell methods: (i) the quantitative evaluation of the co-expression levels of nine different genes, with a plating efficiency of 99-100% and a ...

CD4(-) CD8(-) (double-negative [DN]) T cells have recently been shown to display important immunological functions in human diseases. They express γδ or αβ T-cell receptors that recognize lipid/glycolipid antigens presented via the nonclassical major histocompatibility complex molecules of the CD1 family. We recently demonstrated that while αβ DN T cells serve primarily to express inflammatory ...

Neurexins are cell adhesion molecules involved in synaptic formation and synaptic transmission. Mutations in neurexin genes are linked to autism spectrum disorders (ASDs), which are frequently associated with sleep problems. However, the role of neurexin-mediated synaptic transmission in sleep regulation is unclear. Here, we show that lack of the Drosophila α-neurexin homolog significantly redu...

The plasmid pGKE75-catA138T, which comprises pUC18 and the catA138T gene encoding thermostable chloramphenicol acetyltransferase with an A138T amino acid replacement (CATA138T), serves as an Escherichia coli-Geobacillus kaustophilus shuttle plasmid that confers moderate chloramphenicol resistance on G. kaustophilus HTA426. The present study examined the thermoadaptation-directed mutagenesis of ...

The first TCR-dependent checkpoint in the thymus determines αβ versus γδ T lineage fate and sets the stage for later T cell differentiation decisions. We had previously shown that early T cells in NOD mice that are unable to rearrange a TCR exhibit a defect in checkpoint enforcement at this stage. To determine if T cell progenitors from wild-type NOD mice also exhibit cell-autonomous defects in...

An R-phycoerythrin (R-PE) was isolated by gel filtrations on Sepharose CL-4B and Sephadex G-150 from the phycobiliprotein extract of the marine red macroalga Polysiphonia urceolata Grev and further purified by ion exchange chromatography on DEAE-Sepharose Fast Flow. The purified R-PE showed three absorption peaks at 498 nm, 538 nm, 566 nm and one fluorescent emission maximum at 577 nm. Although...

Cyclically Indecomposable Triple Systems that are Decomposable Martin Grüttmüller Institut für Mathematik, Universität Rostock In this talk, we construct, by using Skolem-type and Rosa-type sequences, cyclically indecomposable two-fold triple systems TS2(v) for all admissible orders. We also investigate exhaustively the cyclically indecomposable triple systems TSλ(v) for λ = 2, v ≤ 33 and λ = 3...

The main objective of this work is to introduce a new type non-linear contraction via C-class functions by using ?-admissible mapping. Our results extend and generalize the very recent Karapinar Sadarangani (2015. RACSAM. [37]). Illustrative examples are given support our findings. We have shown that satisfy periodic fixed point after modifying contraction. Next, we findings from self-mapping T...

According to [LO], the Prym variety of any non-cyclic étale triple cover f : Y → X of a smooth curve X of genus 2 is a Jacobian variety of dimension 2. This gives a map from the moduli space of such covers to the moduli space of Jacobian varieties of dimension 2. We extend this map to a proper map Pr of a moduli space S3M̃2 of admissible S3-covers of genus 7 to the moduli space A2 of principally...