Single-molecule fluorescence spectroscopy addresses biological mechanisms and enables ultrasensitive diagnostics. We describe a new family of single-molecule fluorescence methods that uses alternating-laser excitation (ALEX) of diffusing or immobilized biomolecules to study their structure, interactions, and dynamics. This is accomplished using ratios that report on the distance between and the...

UNLABELLED Laser in situ keratomileusis (LASIK) articles published between 2008 and 2015 that contain clinical outcomes data were reviewed and graded for quality, impression, and potential bias. All 97 relevant articles (representing 67 893 eyes) provided a positive or neutral impression of LASIK. Industry bias was not evident. The aggregate loss of 2 or more lines of corrected distance visual ...

trations after oral dosing. The infection was successfully eradicated with preservation of ambulatory vision. This first case of postoperative VRE endophthalmitis is an unfortunate milestone in ophthalmic surgery. Future cases are probably inevitable given the trends in the epidemiology of VRE infection and colonization, especially in patients with compromised immunity. Colonized donor corneal ...

There has been the unsubstantiated clinical impression that laser refractive surgery accelerates cataract development along with solid experimental data about the cataractogenic effects of excimer laser treatment. We present the first documented case of significant cataract formation in a young myope after repeat excimer laser ablation necessitating phacoemulsification with a posterior chamber ...

Multiple posterior segment complications can occur after LASIK. Posterior vitreous detachment, macular holes, retinal hemorrhages, retinal detachment, and several other complications have been described. A case of posterior vitreous detachment with epiretinal membrane in a young adult after LASIK is reported. LASIK surgeons must be aware of the possibility of posterior segment complications aft...

PURPOSE To describe and evaluate a new multiple regression-derived nomogram for myopic wavefront laser in situ keratomileusis (LASIK). SETTING Moorfields Eye Hospital, London, United Kingdom. DESIGN Prospective comparative case series. METHODS Multiple regression modeling was used to derive a simplified formula for adjusting attempted spherical correction in myopic LASIK. An adaptation of...

OBJECTIVE To evaluate human corneas after laser-assisted in situ keratomileusis at different postoperative intervals. METHODS Thirty-eight postmortem corneas from 20 patients with postoperative intervals from 2 months to 6.5 years after laser-assisted in situ keratomileusis surgery were collected from eye banks. The corneas were trisected and processed for conventional histologic analysis, tr...

purpose: to report a case of diffuse lamellar keratitis (dlk) 9.5 months after laser in situ keratomileusis (lasik) associated with a corneal foreign body. patient and findings: a 32-year-old male developed dlk two days after removal of an intraepithelial corneal foreign body 9.5 months after noncomplicated lasik. dlk was eliminated after two weeks of topical steroid application and visual acui...