flow cytometry (fcm) was used to determine the ploidy levels of six different populationsof agropyron cristatum (l.) gaertn. (poaceae) in iran, using a diploid cultivated barley (hordeumvulgare l.) as an internal reference. according to flow cytometric data, tetraploid (2n = ± 28) andhexaploid (2n = ± 42) levels were detected among the examined populations and these results weresupported by chr...

mitochondrial functions have a major impact on t-cell functionality. in this study we characterized whether mitochondrial function in the neonatal t-cells differs from that in the adult t-cells during short t-cell activation. methods: we used fow cytometry methods to test mitochondrial mass and to monitor mitochondrial ca2+ levels, mitochondrial potential and superoxide generation in parallel w...

Cyclins are key components of the cell cycle progression machinery. They activate their partner cyclin-dependent kinases (CDKs) and possibly target them to respective substrate proteins within the cell. CDK-mediated phosphorylation of specific sets of proteins drives the cell through particular phases or checkpoints of the cell cycle. During unperturbed growth of normal cells, the timing of exp...

Cytokine flow cytometry (CFC) is a general term that applies to flow cytometric analysis of cells using anticytokine antibodies as markers of activation. The most common version of this technique is the intracellular staining of cytokines in cells that have been fixed and permeabilized after short-term in vitro activation. When used with specific antigens, this technique allows for the quantita...

Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing, and analyzing flow cytometry data using extensive computational resources and tools. Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning. Flow cytometry a...

Cellular measurements by flow cytometric analysis constitute an important step toward understanding individual attributes within a population of cells. Assessing individual cells within a population by protein expression using fluorescently labeled antibodies and other fluorescent probes can identify cellular patterns. The technology for accurately identifying subtle changes in protein expressi...

Background: Chronic myelogenous leukemia (CML) is a myeloproliferative disorder due to BCR-ABL1 translocation. Patients showing transformation into blast crisis (BC) have poorer treatment response and prognosis.  Methods: A retrospective study was conducted in the department of Pathology, Maulana Azad Medical College, New Delhi, India, over a time period of 5 years (2014-2019) to evaluate the ...

Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...

The title of this book, Practical Flow Cytometry, may be somewhat misleading; a significant portion of the information presented is not essential to most investigators who utilize flow cytometry. The author's broad expertise in electronics, optics, histochemistry, and biology provides an excellent basis for discussion of the principles of flow cytometry and cell sorting; however, successful app...

Figure 1: Schematic of the particles in the fluid flow and interaction region of the laser within the flow cell. A key and continuing goal in the development of flow cytometry techniques is the ability to measure ever more parameters for each particle under test. Work carried out by Prof John Nolan’s group at La Jolla Bioengineering Institute, and reported recently by Watson et. al.[1] in Cytom...