the cytotoxic activity of natural killer cells is usually tested by radioactive assay csi cr release assay), which detects the release of cytoplasmic contents after plasma membrane disintegration of dying cells. in contrast to this indirect evaluation of cytotoxicity, the assessment of cell damage by flow cytometry aims to provide a more exact characterization of the death pathway via detection...

Breast Cancer is the second-highest prevalence of cancer disease in Indonesia, with number new cases and deaths continues to increase. Kirinyuh or Austroeupatorium inulaefolium (Kunth) R. D. King & H. Robinson a shrub plant, which are widely grown Indonesia. Traditionally, it used for woundhealing information about anticancer potential A. still very limited. This study aims assess cytotoxic act...

The purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-CdA) in the human breast cancer cell line, MDA-MB468 (estrogen receptor negative, ER−). MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay, annexin V-Fluorescein/PI and Hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

Phytochemical investigations of Piper sarmentosum Roxb., yielded four compounds; three amides, identified as 3-(3’,4’,5’-trimethoxyphenylpropanoyl) pyrrolidine, 3-(4’-methoxyphenylpropanoyl) pyrrole, N -(3-phenylpropanoyl) pyrrole and a sterol namely β -sitosterol . 3-(4’-Methoxyphenylpropanoyl) pyrrole was found for the first time in this Piper spe...

Background: One of the important materials needed for root treatment is sealers. They fill space between gutta and canal walls create a suitable seal to prevent colonization oral microorganisms in peri-apical tissues inside space. Aim: The aim this study was prepare test cell biocompatibility new polycaprolactone-based endodontics sealer comparison AH plus against dental pulp stem cells. Method...

background and objectives:scrophularia variegata m. beib. (scrophulariaceae) is a medicinal plant, used for various inflammatory diseases in iranian traditional medicine. in the present study, we evaluated the immune modulation and antioxidant effects of the hydroalcoholic extract of s.  variegata. methods: the splenocytes were harvested from the spleen of balb/c mice and were cultured. the spl...

The implications of disinfection by-products (DBPs) present in drinking water are of public health concern because of their potential mutagenic, carcinogenic and other toxic effects on humans. In this study, we selected 13 main DBPs found in drinking water to quantitatively analyse their cytotoxicity and genotoxicity using a microplate-based cytotoxicity assay and a developed SOS/umu assay in S...

cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-cda) on the breast cancer cell line, mcf-7 (estrogen receptor positive, er+). mtt assay, annexin v-fluorescein/pi and hoechst 33...

Objective: Anticancer activities of Schiff bases S1 and S2 against MG-63 human osteosarcoma cells were performed described using an in vitro evaluation employing cytotoxicity apoptosis assay. Methods: used MTT assay to examine the effect compound on cell viability (S1 S2). Cell morphologies IC50 values obtained. The acridine orange (AO)/ethidium bromide (EB) dual staining technique was determin...

A new live cell-based assay platform has been developed for the determination of complement dependent cytotoxicity (CDC), antibody dependent cellular cytotoxicity (ADCC), and overall cytotoxicity in human whole blood. In these assays, the targeted tumor cell populations are first labeled with fluorescent Cell Tracker dyes and immobilized using a DNA-based adhesion technique. This allows the fac...