NK cell cytotoxicity against the erythroleukaemia cell line K562 was studied, during and after the in vitro exposure to clinically-used concentrations of the general anaesthetic agents nitrous oxide, halothane and enflurane. Each of these agents produced a dose-dependent reduction in cytotoxicity. At approximately equipotent doses nitrous oxide produced less suppression than either halothane or...

MHC class I-related chain molecules A and B (MICA and B) expressed on the cell-surface of tumor cells are ligands for an activating receptor, NKG2D, expressed on natural killer (NK) cells and stimulate the NK cell-mediated cytotoxicity. On the other hand, the soluble form of MICA and B produced by proteolytic cleavage of cell-surface MIC interferes with NK cell-mediated cytotoxicity. We investi...

Measuring the potentially adverse effects of materials on human body is vital and essential for their use, including medical applications. One properties that need to be known cytotoxic effect avoid local systemic effects. It very important scientists professionals are aware body. This study also summarizes data morphology, chemical composition, ethylene-vinyl acetate (EVA) orthopaedic cytotoxi...

Mycophenolic acid (MPA) is the active metabolite of mycophenolate mofetil. This study designed to investigate the mechanism of cytotoxicity of MPA on the genetically engineered PC12 Tet Off (PTO) neuronal cells with p53 gene. Alamar Blue (AB) reduction showed concentration-dependent cytotoxicity of MPA on PTO cells with IC50 value of 32.32 ± 4.61 mM. The reactive oxygen species (ROS) generation...

Glutathione (GSH) has been shown to modulate the Cytotoxicity of a variety of chemotherapeutic agents. The effect of mitomycin C (MMC) treatment duration and the effect of GSH depletion on in vitro cytotoxicity against the human colon cancer cell line 111-29 »asstudied under aerobic conditions. Continuous-exposure experiments revealed that the Cytotoxicity of 0.1 JIMMMC, as measured by clonoge...

Glutathione (GSH) has been shown to modulate the cytotoxicity of a variety of chemotherapeutic agents. The effect of mitomycin C (MMC) treatment duration and the effect of GSH depletion on in vitro cytotoxicity against the human colon cancer cell line HT-29 was studied under aerobic conditions. Continuous-exposure experiments revealed that the cytotoxicity of 0.1 microM MMC, as measured by clon...

The aim of this study was to determine the effects a pin-to-plate cold atmospheric plasma (CAP) on U-251 MG three-dimensional (3D) glioblastoma spheroids under different conditions. 3D tumorspheres showed higher resistance CAP treatment compared 2D monolayer cells. A single able induce cytotoxicity, while multiple treatments augmented effect. also cytotoxicity throughout tumoursphere, and we id...

the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

the effect of natural killer (nk) cells and lymphokine activated killer ( lak) cells was studied on a group of human melanoma cell lines. peripheral blood from healthy volunteers was utilized as a fresh source of natural killer cells and rhi l-2 for producing lak cells. the cytotoxicity of effector cells was quantified using a 4 hour si determining the density of antigen expression on tumor cel...