ent conccntrations ef 2,4-D, NAA and BAP, large number of somatic cmbryos differentiated When the suspension was cultured for 7 days in the induction medium. The cel]s destined to become somatic embryos divided to form spherical of filamentous proembryos. The fi]amenteus proembryo developed into different shapes of embryos. Somatic embryogenesis began in tlie proembryo stage and preceeded from ...

to produce embryogenic callus and somatic embryos, tendril explants were collected and cultured in ms medium supplemented with various concentrations of hormones. after one month most of explants produced callus. the highest percent of embryogenic callus obtained in ms medium supplemented with 4.5 µm bap and 1µm 2,4-d. production of somatic embryos was depended on genotype and medium. yaghouti ...

A method for tissue culture of micropropagation aquatic plant Staurogyne repens (Nees) Kuntze has been developed. Different phytohormones were investigated to optimize the growth explants. The shoot tip explants S. placed in MS nutrient medium including BAP (0.30-1.50 mg/l) + TDZ (0.10 NAA and Kn mg/l). Shoot regeneration frequencies changed as 77.77-100% 66.66-100% cultivation augmented with B...

Origanum acutidens (HAND.-MAZZ.) IETSWAART is a promising ornamental plant that can be widely used in landscape management. It is endemic to Eastern Anatolian region of Turkey. Tissue culture has not been used to micropropagate it. The study reports stem node explants from one-week-old seedlings of the plant for successful micropropagation. The stem nodes were cultured on MS medium containing 0...

A protocol for regeneration of an endangered medicinal orchid Dendrobium crepidatum was established mainly via indirect protocorm like body (PLBs) formation. The PLBs procured from in vitro growth cultures assessed ½ MS medium alone and combinations with 6-Benzyl amino purine (BAP), Kinetin (Kn) α-Naphthalene acetic acid (NAA). calli were induced longitudinally bisected segments PLBs. optimum p...

BACKGROUND AND AIMS Cymbidium mastersii is an epiphytic orchid distributed mainly in Northeast India. Owing to its high commercial value in the floricultural industry, natural populations are under threat from over-exploitation. Mass propagation provides an alternative means of satisfying the demand. Unfortunately, conventional propagation is slow and difficult, suggesting in vitro methods for ...

Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro of C. been successfully established but somaclonal variation was not evaluated. The pathway and number subcultures on were evaluated using six ISSR primers amplified 58 loci Inter Simple Sequence Repeats (ISSR). A dendrogram based Jaccard´s genetic distance...