Background: Inventory management of platelets is critically important to avoid shortage and minimise wastage.1 In our centre this challenging with a platelet order delivery time 12–24 hours no other stock within 400 km radius. Aim: To examine if phoning clinical haematology staff reduced wastage. Methods: Our senior transfusion scientist introduced routine calls when were expiring in the next 1...

A method to measure DNA and chlorophyll fluorescence simultaneously in single phytoplankton cells was developed and tested on cultured and natural populations. Samples were stored at -80°C following fixation with 1 % glutaraldehyde and freezing in Ilquid nitrogen, stained with DAPI (4',6-&amino-2-phenylindole), and analyzed by flow cytonletry. In cultures, cell DAPI-DNA fluorescence measured by...

The karyotypes of four South American species of Cestrum (C. capsulare,C. corymbosum,C. laevigatum and C. megalophylum) were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metacentric chromosomes, except for the eighth submeta- to acrocentric pair. Several types of heterochr...

Simple, efficient, and safe tagging methods are desired in short-term microbial transport studies such as in the study of filtration systems for water and wastewater treatment. Suitability of selected fluorochromes as bacterial tagging agents in transport studies was evaluated on the basis of stability of stained cells and the effect of staining on bacterial surface characteristics and interact...

Turnera sidoides is an autopolyploid complex of obligate outcrossing perennial herbs. It includes five subspecies and five morphotypes in which diploid to octoploid cytotypes were found. Based on phenetic analyses of the complex and karyotype data of polyploid cytotypes, it has been hypothesized that morphological and chromosome differentiation of T. sidoides occurred at the diploid level. To t...

Thiamine (vitamin B1), which is synthesized only in bacteria, fungi and plants humans should take with diet, participates basic biochemical physiological processes a versatile way its deficiency associated neurological problems accompanied by cognitive dysfunctions. The rat glioblastoma (C6) model was used, exposed to limited environment toxicity glutamate. cells were stressed exposure glutamat...

Fishes of the Loricariidae family, known as "cascudos", constitute an endemic group in Neotropical freshwaters. In this study, were cytogenetically examined two species of Loricariidae (Pterygoplichthysanisitsi Eigenmann & Kennedy, 1903 and Farlowellaamazonum (Günther, 1864) belonging to Hypostominae and Loricariinae subfamilies respectively) from Iguatemi River. Our study provide the first des...

One hundred and seven healthy volunteers were administered Cloninger's Tridimensional Personality Questionnaire (TPQ), the Differential Attentional Processes Inventory (DAPI), the Tellegen Absorption Scale (TAS), and the Stanford Hypnotic Susceptibility Scale, Form C (SHSS:C). Polymorphisms of catechol O-methyltransferase (COMT), an enzyme involved in dopamine metabolism, were assessed. Highly ...

DNA anaphase bridges are a potential source of genome instability that may lead to chromosome breakage or nondisjunction during mitosis. Two classes of anaphase bridges can be distinguished: DAPI-positive chromatin bridges and DAPI-negative ultrafine DNA bridges (UFBs). Here, we establish budding yeast Saccharomyces cerevisiae and the avian DT40 cell line as model systems for studying DNA anaph...