نتایج جستجو برای: dehalogenases
تعداد نتایج: 191 فیلتر نتایج به سال:
UNLABELLED The epoxide hydrolases and haloalkane dehalogenases database (EH/HD) integrates sequence and structure of a highly diverse protein family, including mainly the Asp-hydrolases of EHs and HDs but also proteins, such as Ser-hydrolases non-heme peroxidases, prolyl iminopetidases and 2-hydroxymuconic semialdehyde hydrolases. These proteins have a highly conserved structure, but display a ...
Current study was to investigate the presence of dehalogenase in isolated bacterium from Selat Tebrau that can grow on 2,2-dichloropropionic acid (2,2-DCP is an active compound herbicide Dalapon®). Strain RN1, a Gram-negative and rod shape tentatively identified as Enterobacter cancerogenus based basic biochemical 16S rRNA gene analyses. The calculated cells doubling time were 5.29 hours growth...
"Dehalococcoides" bacteria can reductively dehalogenate a wide range of halogenated organic pollutants. In this study, DNA microarrays were used to monitor dynamic changes in the transcriptome as "Dehalococcoides ethenogenes" strain 195 transitioned from exponential growth into stationary phase. In total, 415 nonredundant genes were identified as differentially expressed. As expected, genes inv...
Haloalkane dehalogenase DhmA from Mycobacterium avium N85 showed poor expression and low stability when produced in Escherichia coli. Here, we present expression DhmA in newly constructed pK4RP rhodococcal expression system in a soluble and stable form. Site-directed mutagenesis was used for the identification of a catalytic pentad, which makes up the reaction machinery of all currently known h...
Dehalococcoides ethenogenes is the only bacterium known to reductively dechlorinate the groundwater pollutants, tetrachloroethene (PCE) and trichloroethene, to ethene. Its 1,469,720-base pair chromosome contains large dynamic duplicated regions and integrated elements. Genes encoding 17 putative reductive dehalogenases, nearly all of which were adjacent to genes for transcription regulators, an...
The enzyme DhaA from Rhodococcus rhodochrous NCIMB 13064 belongs to the haloalkane dehalogenases, which catalyze the hydrolysis of haloalkanes to the corresponding alcohols. The haloalkane dehalogenase DhaA and its variants can be used to detoxify the industrial pollutant 1,2,3-trichloropropane (TCP). Three mutants named DhaA04, DhaA14 and DhaA15 were constructed in order to study the importanc...
Desulfitobacterium hafniense is an anaerobic, low GC, Gram-positive spore-forming bacterium that shows considerable promise as a bioremediative competent population of sediments. Of particular relevance to its remediation capabilities are metal reduction, which changes the mobility and toxicity of metals, and chlororespiration, the ability to dechlorinate organic xenobiotics. Our work focuses o...
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