In this current report, a simple, reliable, and rapid method based on modifying the cellulose surface by doping it with different percentages of lanthanum hydroxide (i.e., 1% La(OH)3-cellulose (LC), 5% La(OH)3-cellulose (LC2), and 10% La(OH)3-cellulose (LC3)) was proposed as a selective marker for detection of copper (Cu(II)) in aqueous medium. Surface properties of the newly modified cellulose...

To obtain purified H and M antigens suitable as primary standards in the serological diagnosis of histoplasmosis by agar gel double-diffusion tests, H and M reactive components of histoplasmin were fractionated by column chromatography by using Sephadex G-100, Sephadex G-200, and diethylaminoethyl cellulose. Six fractions from diethylaminoethyl cellulose were reactive in agar gel double-diffusi...

Crystalline hexokinase prepared by the method of Dar-row and Colowick consists largely of enzyme which has been modified in its chromatographic and electrophoretic behavior by yeast protease which is present during the isolation procedure. This proteolytic modification can be prevented by removal of the protease by chromatography on diethylaminoethyl cellulose; it can also be largely inhibited ...

The cytoplasmic (175,000 x g supernatant) and the chromatin fractions from phytohemagglutinin-stimulated normal human lymphocytes, human thymus tissue, lympho cytes from chronic lymphocytic leukemia and acute lymphoblastic leukemia patients and cultured cells of normal (RPMI 1788), multiple myeloma (RPMI 8226), Burkitt lymphoma (HR1K), and acute lymphoblastic leukemia (Molt-4) origin were exami...

Two polyphenol oxidases (enzymes A and B) from Bartlett pear (Pyrus communis) peelings were purified to electrophoretic homogeneity according to polyacrylamide gel by a combination of Sephadex gel filtration, diethylaminoethyl cellulose chromatography and hydroxyl apatite chromatography. While the two enzymes differ electrophoretically at pH 9.3, chromatographically on hydroxyl apatite, and in ...

As a result of a general study on the partition column chromatography of insulin, it was noted that a number of commercial samples of crystalline insulin gave elution curves which indicated the presence of at least two components in the eluted fractions (3, 4). The solvent systems used in these studies were similar to those that had been reported by Harfenist and Craig in their countercurrent d...

Abstract A chemical modification of cotton fabrics by 2-diethylaminoethyl chloride (DEAE-Cl) was achieved, and the resulted demonstrated salt-free dyeing properties with anionic dyes. Nucleophilic property hydroxyl groups in cellulose enhanced under alkaline conditions could react DEAE-Cl, a possessing both nucleophilic electrophilic sites. The monolayered DEAE-grafted further DEAE-Cl to form m...

Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone fractionation of soluble plasmodial homogenates acidified to pH 5.0. The M-MDH...

Chromatographic techniques have recently been introduced which provide new approaches to serum protein separation and characterization (1-4). Sober, Gutter, Wyckoff, and Peterson, using diethylaminoethyl (DEAE) cellulose, described the initial application of anion-exchange cellulose chromatography to the fractionation and identification of serum proteins (1, 2). Their study indicated the great ...