We have reported that extent of proliferation atypical hepatocytes (POAH) in non-cancerous liver hepatocellular carcinoma and chromatin licensing DNA replication factor 1 (CDT1) are associated with postoperative recurrence. Here, we investigated whether POAH expression CDT1 also chemically induced cancer rats. Male Fisher strain rats were orally administered diethylnitrosamine (DEN) their drink...

Doxorubicin is a broad spectrum antitumor antibiotic. Newcastle disease virus (NDV ) one such with an inherent oncolytic property. This study was done to investigate the effects of and NDV on Diethylnitrosamine (DEN) induced Hepatocellular carcinoma in rats. Seventy five male albino rats were divided into groups. Group (1): administered distilled water only. (2) : received diethylnitrosoamine (...

The monoterpenes d-limonene and perillyl alcohol (POH) inhibit the growth of mammary tumors. In this investigation we tested whether POH is also effective in reducing liver tumor growth. Diethylnitrosamine was used to induce liver tumors in male Fischer 344 rats. Two weeks after diethylnitrosamine exposure was discontinued, the animals were divided into POH-treated and untreated groups. The mea...

Chemically induced rat bepatocyte nodules and carcinomas have a reduced capacity to oxidize drugs. The reduction in monooxygenase activity results largely from the partial loss of cytochrome P-450, a hemecontaining terminal electron acceptor. To determine whether the cyto chrome P-450 deficit was indicative of an altered heme metabolism, we quantitated four heme-containing proteins in normal ra...

The chemopreventive effects of decaffeinated green and black tea treatment on liver and lung tumorigenesis were examined in carcinogen-treated mice. Male C3H mice were given decaffeinated green or decaffeinated black tea in their drinking water prior to, during, and after treatment with diethylnitrosamine (50 micrograms/kg bw, i.p., once per week for 8 weeks). After 40 weeks of tea treatment, m...

Experiments were performed to assess the role of liver cell necrosis in the induction of early steps in liver carcinogenesis with diethylnitrosamine, as measured by the appearance of foci of resistant hepatocytes that stain for y-glutamyl transpeptidase and that are presumptive preneoplastic lesions in the rat. With the use of a necrogenic dose of diethylnitrosamine and an assay for the carcino...

Diethylnitrosamine (DEN), found in many commonly consumed foods, is widely reported to induce cancer in animals and humans. The aim of the present study was to investigate the hepatoprotective and antioxidant activities of the leaf extract of the medicinal plant Cassia fistula Linn. against diethylnitrosamine induced liver injury in ethanol pretreated rats. Albino Wistar rats, pretreated with e...

BACKGROUND AND AIM Aberrant activation of the TGF-β1/Smad pathway contributes to the activation of hepatic stellate cells (HSCs). MicroRNA-195 has been shown to regulate the activation of HSCs. The aim of this study was to investigate the role of miRNA-195 in HSCs activation. METHODS A liver fibrotic rat model induced by diethylnitrosamine was established. Dual luciferase reporter assays were...