Oral staining can be secondary to intrinsic or extrinsic environmental factors [1]. Extrinsic is recognized mostly on dental surfaces and commonly associated with the use of chlorhexidine mouthwash [2,3,4], certain toothpastes [5], frequent intakes tea and/or coffee [6,7]. When discoloration appears a mucosal surface, clinician should careful take thorough history build differential diagnosis b...

objective(s) the main objective of this study was to investigate the status of chromosome stability in 3 human-mouse hybridoma cell lines over a period of time in various passages. materials and methods metaphase spreads from 3 human-mouse cell lines (hf2x653, spmo-4 and f3b6) that had been cultured in 4 successive passages, from 1 to 4 weeks, were prepared and analyzed. metaphase chromosomes s...

hbme-l is an antimesothelial monoclonal antibody that recognizes an unknown antigen on microvilli of mesothelial cells. the antibody is only relatively specific for mesothelium and is used in the differential diagnosis of mesothelioma and adenocarcinoma within the context of an appropriate immuno-histochemical panel. hbme-l has also been reported to strongly and uniformly stain papillary and fo...

Microscopic discrimination between extracellular and invasive, intracellular bacteria is a valuable technique in microbiology and immunology. We describe a novel fluorescence staining protocol, called FITC-biotin-avidin (FBA) staining, which allows the differentiation between extracellular and intracellular bacteria and is independent of specific antibodies directed against the microorganisms. ...

background: lymphadenitis is a common form of extra-pulmonary tuberculosis. as in peripheral health centers, the facili­ties for histopathology and culture are lacking, material obtained by fine needle aspiration (fna) in suspected cases of tubercu­lar lymphadenitis (tbl) cid fast bacilli (afb) by ziehl neelson method as every designated micros­copy centre under revised national tuberculosis co...

background : cd117 is a thyrosin kinase receptor encoded by c-kit proto-oncogene. it is expressed during normal development in some tissues and also in a subset of neoplasia especially gastrointestinal stromal tumors   (gists). treatment with thyrosin kinase inhibitors (e.g., imatinib) is useful in cd117- positive gists. the goal of this study is to investigate the expression of cd117 in glial ...

argyrophilic staining of nucleolar organizer regions (agnor) has been considered to be useful in the diagnosis and prognostic evaluation of different cutaneous tumors. in order to evaluate the role of the agnor technique in discriminating aggressive from non-aggressive basal cell carcinoma (bcc), paraffin-embedded histologic sections from 30 cases of aggressive bcc (bcc2) and 30 cases of non-ag...

nucleolar organizer regions (nor) are defined as nucleolar components containing a set of argyrophilic proteins which are selectively stained by colloidal silver nitrate staining. although studies have shown that the number of nor dots or particles is directly related to the rapidity of cell proliferation in cancer cells, prognostic or diagnostic value of nor remains controversial. the aim of t...

background: tuberculosis continues to be a serious public health problem causing nearly three million deaths per year all over the world. despite major improvement in diagnosis, it is not possible to control the disease in the absence of surveillance treatment, and follow-up programs. objectives: this research was designed to study the frequency of mycobacterium tuberculosis among specimens ref...

The protein insulin-like growth factor II mRNA binding protein 3 (IMP3) is an important factor for cell migration and adhesion in malignancies. Recent studies have shown a remarkable overexpression of IMP3 in different human malignant neoplasms and also revealed it as an important prognostic marker in some tumor entities. The purpose of this study is to compare IMP3 immunostaining in squamous c...