An enzyme-linked immunosorbent assay (ELISA) was developed on the basis of testing a single dilution of serum to quantify the level of antibody to the p57 protein of Renibacterium salmoninarum in sockeye salmon (Oncorhynchus nerka). The levels of antibody were interpolated from a standard curve constructed by relating the optical densities (OD) produced by several dilutions of a high-titer rain...

The purpose of the present study has been to determine the change in the resistance of corpuscles to saponin with changes in pII., the acids employed being the common inorganic acids. Ponder (1) has shown that the rate of saponin hemolysis is increased in the presence of certain acids and has applied the equation Cl = R (C, + g) to systems in which an acid is the accelerating agent. Details of ...

AT THE PRESENT time, indicator-dilution curves are one of the most important technics employed in the investigation of the circulation. The earliest of these studies were carried out by the rapid serial collections of blood samples and their subsequent photometric analysis.' This ingenious but cumbersome method was replaced by the continuous withdrawal of blood in front of a photoelectric cell,...

Abstract The critical nitrogen (N) dilution curve, which expresses whole-plant N concentration as a function of shoot biomass, can be used management diagnostic tool for cereals. objectives this research were to develop curve wheat grown in calcareous soils and formulate model estimating fertilizer requirement crop at different growth stages. Six fertilization rates (0–250 kg ha −1 ) induce var...

AIM The aim of this work is to establish two simple, economical, and rapid spectrophotometric methods for the quantification of entacapone in bulk material and in tablets. Further, this study is designed to validate the developed methods as per ICH guidelines. MATERIALS AND METHODS In Methods I and II, a stock standard solution was prepared by dissolving 10 mg of entacapone in 100 mL of 10% v...

The concept of critical nitrogen (Nc) concentration can be implemented to diagnose in-season plant nitrogen (N) status for optimizing N fertilizer management. The Nc dilution curves have been established for rice (Oryza sativa L.) grown in different climatic regions, yet no attempt has been made to develop the Nc dilution curve for double cropping rice regions. This study was undertaken to deve...

The mechanism of light reflection in blood, which is the underlying principle for the use of fiber optics in in vivo densitometry or oximetry, was investigated theoretically and experimentally using bovine blood. The results are summarized as follows. 1) Theoretically, the intensity of light reflected, I, was represented by a formula: I=lo•~e-2KC•~[(1-P)•~r•~a, where I, K, C, p, and a were inci...

BACKGROUND Major differences in plasma volume expansion between infusion fluids are fairly well known, but there is a lack of methods that express their dynamic properties. Therefore, a closer description enabled by kinetic modeling is presented. METHODS Ten healthy male volunteers received, on different occasions, a constant-rate intravenous infusion over 30 min consisting of 25 ml/kg of 0.9...

BACKGROUND Hypertonic saline hydroxyethyl starch (HH) has been recommended for first line treatment of hemorrhagic shock. Its effects on coagulation are unclear. We studied in vitro effects of HH dilution on whole blood coagulation and platelet function. Furthermore 7.2% hypertonic saline, 6% hydroxyethylstarch (as ingredients of HH), and 0.9% saline solution (as control) were tested in compara...