Biopsy specimens from 21 women with primary operable breast cancer were analyzed in a double blind study designed to compare fluorescent antibody methods for detection of estrogen receptors with estrogen receptors measured biochemically with a previously reported dextran coated charcoal technique (DCC). Fluorescent antibody methoids employed both direct techniques: (1) rabbit antisera to 6-O-Ca...

Aqueous glutaraldehyde has been polymerized under alkaline conditions in the presence of a surfactant to yield microspheres of varied diameters. Microbeads of a nominal 300 nm diameter, made fluorescent via fluoresceinisothiocyanate, were used as a carrier of rabbit anti-human red blood cell antibodies. Specific labeling of the human red blood cells as well as diagnostic use of these fluor...