A use-dilution procedure and screening method are proposed to aid the hospital bacteriologist in selecting the most effective disinfectants. Sixteen disinfectants were tested with and without organic material on six different organisms. Sporadic results usually obtained with quaternaries tested by the procedure of the Association of Official Agricultural Chemists (AOAC) were eliminated by the m...

Environmental and patient isolates of Mycobacterium avium were resistant to chlorine, monochloramine, chlorine dioxide, and ozone. For chlorine, the product of the disinfectant concentration (in parts per million) and the time (in minutes) to 99.9% inactivation for five M. avium strains ranged from 51 to 204. Chlorine susceptibility of cells was the same in washed cultures containing aggregates...

Methods: Disinfectants were prepared according to the manufacturers’ label directions and mixed with virus stock. The disinfectant-virus solution was then passed through a detoxification column to remove compounds toxic to cell culture. The filtrate was collected, serially diluted, and inoculated onto porcine kidney cells (PK15). After a 48-hour incubation period, the cell cultures were fixed a...

Terminal cleaning in the operating room is a critical step in preventing the transmission of health care-associated pathogens. The persistent disinfectant activity of a novel isopropyl alcohol/organofunctional silane solution (ISO) was evaluated in 4 operating rooms after terminal cleaning. Adenosine triphosphate bioluminescence documented a significant difference (P < .048) in surface bioburde...

Agents causing transmissible spongiform encephalopathy (TSE) diseases are resistant to inactivation by several conventional decontamination methods. Using an animal bioassay, we compared the TSE agent disinfectant efficacy of a commercially available product referred to alternatively as LpH-SE, LpH-AG, or LpH-st to that of a similarly named but differently formulated product, Environ LpH, which...

The exact mechanism of action of a disinfectant is not easy to elucidate. The notion of 'target' in the bacterial cell, frequently evoked for the antibiotics, is not clear for disinfectants (except for some, e.g. chlorhexidine). In understanding the mode of action of a disinfectant, it can be difficult to distinguish the primary stage (characteristic of the mode of action) and the secondary sta...

Ozone is a powerful drinking water disinfectant that is effective in treating chlorine resistant organisms, such as Cryptosporidia. For bottled water, ozonation is generally preferred over other available disinfection treatment methods because it does not leave a taste or residual disinfectant, due to ozone’s short lifetime. However, ozonation of drinking water containing bromide can result in ...

The presence of antibiotics in the environment and their subsequent impact on the development of multi-antibiotic resistant bacteria has raised concerns globally. Consequently, much research is focused on a method to produce a better disinfectant. We have established a disinfectant system using UVA-LED that inactivates pathogenic bacteria. We assessed the bactericidal efficiency of a combinatio...

The aim of this study was evaluation an organic germicide (Aquagerm) histological affects on Rainbow trout (Oncorhynchus mykiss) vital tissues. This new disinfectant is an aquatic antiseptic and mixed-oxidant disinfectant which is produced for the first time in Iran. This new product mechanism is based on oxidant properties of pro-sulphate which attack crucial structures of pathogens. In this s...

The chlorophenols have disinfectant-like tastes, detectable in water in concentrations ranging from 0.04 parts per billion (ppb) for 2, 6-dichlorophenol to 30 ppb for pentachlorophenol (PCP). The chlorophenols are soluble in most organic solvents and are slightly soluble in water. Chlorocresols share the disinfectantchemical taste of the chlorophenols, and may have even lower taste threshold va...