Hydrogen peroxide is formed in solutions of glutathione exposed to oxygen. This hydrogen peroxide or its precursors will decrease the viscosity of polymers like desoxyribonucleic acid and sodium alginate. Further knowledge of the mechanism of these chemical effects of oxygen might further the understanding of the biological effects of oxygen. This study deals with the rate of solution of oxygen...

background : the aim of the current study was to develop a marking technique as an internal marker to mark post blood meal mosquitoes by using stable phosphate isotope 32 p and determine the optimal concentration of it. methods : an isotonic physiological saline solution, containing different concentration of radioactive isotope 32 p-labeled disodium phosphate (na 2 h 32 po4) was injected into ...

We have previously presented evidence that acyl dihydroxyacetone phosphate is converted to O-alkyl dihydroxyacetone phosphate via an endiol intermediate which then accepts a fatty alcohol to form O-alkyl dihydroxyacetone phosphate. We have further proposed that, in the absence of fatty alcohol, the endiol derivative of acyl dihydroxyacetone phosphate reacts with water to form dihydroxyacetone p...

A histochemical method for demonstration of glyceraldehyde-3-phosphate dehydrogenation by tissues is described. The method utilizes Nitro BT as an indicator, glyceraldehyde-3-phosphate obtained from hydrolysis of commercially obtainable glyceraldehyde-3-phosphate diethylacetal (monobarium salt) as substrate, and (ethylenediamine)tetraacetic acid acid disodium as an activating agent in a medium ...

1. Previous studies have shown that in thyroparathyroidectomized rats injection of disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP) at doses that inhibit bone mineral retention (0.16 mmol = 10 mg of phosphorus/kg body wt. per day subcutaneously) leads to a decrease in the net tubular reabsorption of phosphate. 2. In the present work the tubular response to EHDP (0.16 mmol/kg body wt.) injecte...

Pulmonary alveolar microlithiasis (PAM) is a rare chronic genetic lung disease in childhood with no proven therapy. It is characterized by the deposition of calcium phosphate microliths within the alveolar air spaces. The effect of disodium etidronate (DE) treatment on PAM is controversial. We report 3 siblings (an 11-year-old boy and 4-year-old twin girls) with PAM diagnosed by chest X-ray, th...

The usual methods for the determination of calcium in serum are lengthy procedures. Calcium may be precipitated as oxalate and the oxalate radical estimated by titration with potassium permanganate (Clark and Collip, 1925). The calcium oxalate may be converted to calcium carbonate which is estimated by titration with acid (Trevan and Bainbridge, 1926). Calcium may also be precipitated as calciu...

A kinetic microspectrophotometric assay for galactose1-phosphate uridylyitransferase (EC 2.7.7. 12) activity in erythrocytes is described. BlOOd is collected in ammonium heparinized microhematocrit tubes, centrifuged and the erythrocytes are lysed with water. Galactose-1-phosphate uridylyltransferase activity is determined by mixing 25 ul of hemolysate with a reagent consisting of galactose1-ph...

Solid-state NMR has been used to analyze the chemical environments of sodium sites in powdered crystalline samples of sodium nucleotide complexes. Three of the studied complexes have been previously characterized structurally by crystallography (disodium deoxycytidine-5'-monophosphate heptahydrate, disodium deoxyuridine-5'-monophosphate pentahydrate and disodium adensoine-5'-triphosphate trihyd...