Water intake reduction (anti-dipsogenic effects) under hypoxia has been well established, but the underlying reason remains unknown. Our previous report indicated that activated TRPV4 neurons in SFO are associated with anti-dipsogenic effects under hypoxia. Although low partial pressure of blood oxygen directly activates TRPV4, humoral factors could also be involved. In the present study, we hy...

The lamina terminalis was severed by a horizontal knife cut through the anterior commissure to determine the effects of a disconnection of the subfornical organ (SFO) on drinking and Fos-like immunoreactivity (Fos-ir) in the rat brain in response to an intragastric load of hypertonic saline (5 ml/kg of 1.5 M NaCl by gavage). After an initial load, knife-cut rats drank significantly less water t...

The subfornical organ (SFO) and the periventricular tissue of the anteroventral third ventricle (AV3V) have been shown to be important for the central action of circulating angiotensin. Recent anatomical findings have elucidated neural connections between the SFO and several structures within the AV3V region. The present study examined the function of fibers between the SFO and the median preop...

The subfornical organ (SFO) is sensitive to both ANG II and ACh, and local application of these agents produces dipsogenic responses and vasopressin release. The present study examined the effects of cholinergic drugs, ANG II, and increased extracellular osmolarity on dissociated, cultured cells of the SFO that were retrogradely labeled from the supraoptic nucleus. The effects were measured as ...

A lesion of the subfornical organ (SFO) may disrupt drinking after a meal of dry chow as it does drinking after intragastric administration of hypertonic saline. Food and water intakes of SFO-lesioned (SFOX) and sham-lesioned rats were measured during 90-min tests following various lengths of food deprivation. During the tests, all rats began eating before they began drinking. After 20-24 h of ...

Dispersive Liquid-Liquid MicroExtraction technique based on Solidification of a Floating Organic drop (DLLME-SFO) combined with flame atomic absorption spectrometry was developed for determination of thallium in water sample. An appropriate mixture of acetone and 1-undecanol was rapidly injected into an aqueous sample containing TlCl4- which forms an ion pair with bril...

the antibiotic residues in milk are a well-known serious problem and pose several health hazards to consumers. we have described a simple, rapid, and inexpensive dllme-hplc/uv technique for the extraction of chloramphenicol and florfenicol residues in milk samples. under the optimum conditions, linearity of the method was observed over the range 0.02-0.85 µg/l with correlation coefficients > 0....

It has been 30 years since landmark studies by Johnson and Brody1 revealed a pivotal role for the forebrain circumventricular organs (CVOs) in experimental hypertension. We have since come to understand that these small midline structures, mounted along the cerebral ventricles and lacking well-formed blood-brain barriers, shoulder a huge responsibility for maintaining the delicate balance of ca...

Mineral water is known for its high purity. Because mineral water is produced by the infiltration of water through the soil, there is the possibility of contamination with pesticides. The aim of this study was to develop a simple, rapid and efficient method for the extraction and preconcentration of different classes of pesticides in mineral water samples by dispersive liquid-liquid microextrac...