BACKGROUND Traditionally, for DNA analyses, DNA is recovered from buffy coats. Since DNA in urine has been reported to deteriorate quickly, this option is often not considered. To complete our DNA database in patients with ANCA-associated vasculitis, we aimed to extract DNA from stored urine. METHODS Urine was stored at the time of kidney biopsy from patients included in our regional kidney b...

Herbarium collections are potentially an enormous resource for DNA studies, but the use of herbarium specimens in molecular studies has thus far been slowed down by difficulty in obtaining amplifiable DNA. Here we compare a set of commercially available DNA extraction protocols and their performance in terms of DNA purity and yield, and PCR amplification success as measured by using three diffe...

Analysis of purity and concentration isolated DNA is a way to see the quality DNA. This research was conducted as an initial stage give. Information about in future research. The purpose this provide references information regarding isolation techniques chondroitin samples, so that they can be used similar studies. sample consisted which weighed 50 mg tested 10 times. method study centrifuge co...

The sensitivity and reliability of PCR for diagnostic and research purposes require efficient unbiased procedures of extraction and purification of nucleic acids. One of the major limitations of PCR-based tests is the inhibition of the amplification process by substances present in clinical samples. This study used specimens spiked with a known amount of plasmid pBKV (ATCC 33-1) to compare six ...

extraction of intact quality dna from plant tissues, especially those rich in secondary metabolites, is often challenging. literally, hundreds of different dna isolation protocols from various plant species have been published over the last decades. although many commercial dna isolation kits are convenient and designed to be safe, their cost and availability cause limitations in small molecula...

Forensic experts in uncovering the truth of a case must go through identification, documentation, and collection evidence. Sometimes at crime scene, lip prints are found on surface an object such as mouth plastic bottle. Lip print research bottle mouths was carried out for 1, 3 7 days. There 21 samples with details controls, 6 first day, 3rd 7th day. DNA extraction using DNAzol, quantification ...

Analysis of the purity and concentration DNA isolation results was carried out using samples dragonflies (Onychogomphus forcipatus) species insects. This research is important because there are many kinds in molecular field that require good techniques to support success research. The purpose this paper provide information about quality isolated seen parameters. extraction method uses centrifug...