Recently the dogma that there is an inverse linear association between androgen receptor (AR) CAG and GGN polymorphisms and receptor activity has been challenged. We analysed the pattern of association between 21 male reproductive phenotypes and AR CAG/GGN repeat lengths in 557 proven-fertile men. A linear association was only found between sperm DNA fragmentation index (DFI) and CAG length, an...

background: the aim of the study was to assess the effect of four repeated ejaculations on the same day at two-hour intervals on conventional and functional semen parameters. methods: three healthy men (32±3.6 years) donated the first semen samples after 3-4 days of sexual abstinence followed by three subsequent samples on the same day at two-hour interval each. semen samples were processed and...

Abstract Study question In men with Raised Sperm DNA Fragmentation Index (DFI), Would advance sperm preparation techniques at Intra-Cytoplasmic Injection (ICSI) alter embryo euploidy status? Summary answer raised DFI, use of selection are safe and do not appear to ploidy status the embryos What is known already MACS (Magnetic Activated Cell Sorting), Microfluidic Sorting (MF), surgically retrie...

Abstract Background The routine semen analysis fails to detect sperm DNA damage which contributes the majority of male factor infertility. Sperm fragmentation test (DFI) measures damage. Blastocyst formation is an important step in IVF ± ICSI. At present, literature lacks any data that correlates DFI and blastocyst formation. Main body abstract We searched MEDLINE other databases till 2020 for ...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Dr. Oehninger’s elegant editorial dwelled on several unresolved i s sues in the context of sperm DNA fragmentation (SDF) (1). Indeed, we appreciate and agree with his comments that development of better diagnostic methodology, evaluation of true functional consequences and understanding of oocyte repair capacity are i...

Female factors are very important in determining the success of vitro fertilization (IVF). However, many reports have shown that male also affect or failure IVF. Therefore, sperm evaluation is important, semen examination criteria based on conventional methods alone not a primary factor consideration for IVF embryonic potential, pregnancy rates, and miscarriage rates after transplantation. We p...

Abstract Study question Which sperm preparation procedure is able to select the best population of sperm? Summary answer Microfluidic sorting using Fertile Plus device isolates with highest motility and lowest levels DNA fragmentation. What known already The selection in Medically Assisted Reproduction should lead fully functional spermatozoa reach fertilization site allow optimal embryo develo...

Abstract Study question Does sperm DNA fragmentation (SDF) by TUNEL affect reproductive success measured as live birth rate (LBR) in egg recipients after euploid embryo transfer? Summary answer SDF increases miscarriage rates with PGT-A. Therefore, depends on damage transfer. What is known already Sperm integrity important for optimal fertilisation, implantation and pregnancy. Although controve...

Abstract Study question Does short ejaculatory abstinence time (EA) improve DNA fragmentation and reproductive outcomes compared with long EA following assisted technology (ART)? Summary answer Shorter is associated improved pregnancy live birth rates ART lower DFI longer at different cut-off points. What known already When spermatozoa pass the epididymal tract, they are exposed to reactive oxy...

This study compares the relative effects of advancing male age on multiple genomic defects in human sperm [DNA fragmentation index (DFI), chromatin integrity, gene mutations, and numerical chromosomal abnormalities], characterizes the relationships among these defects and with semen quality, and estimates the incidence of susceptible individuals for a well characterized nonclinical nonsmoking g...