HE genetic factor responsible for the depression of the frequency of streptomycin-resistance transformations in Pneumococcus was first studied by GREEN (1959). He found that when Rx cells are exposed to a DNA transforming preparation (TP) containing genetic markers for erythromycin resistance and streptomycin resistance, approximately identical frequencies of transformation by both markers are ...

The complex genomic structure of eukaryotic cells is primarily achieved by the binding DNA to histones. Different members histone families form a with and, as nucleosome, constitute functional unit chromatin. In addition their structural functionality, histones are also involved in other molecular mechanisms, such damage recognition and repair. A very important factor management H2A.X. phosphor...

Chrysanthemum morifolium is one of the most popular ornamental plants in world. However, as C. a segmental hexaploid, self-incompatible, and has sizable heterologous genome, it difficult to modify its trait systematically. Genome editing technology attractive methods for modifying traits For commercial use genetically modified morifolium, rigorous stabilization quality essential. This stability...

Background: Ovarian carcinoma is one of the leading causes of cancer-related death among females. K-ras codon 12 mutations are commonly occurring mutations in different types of cancers and leads to resistance against anti-EGFR therapeutics. Hence, determination of mutations in k-ras gene is crucial for predicting response to anti-EGFR therapies. This study aimed to evaluate the prevalence of k...

Sumba Ongole (SO) cattle are from India which were imported to Indonesia during the Dutch colonial era and well adapted on Island, East Nusa Tenggara, Indonesia. known for high meat production. This research was conducted identify one point mutation of g.415C/A (F94L marker) in myostatin (MSTN) gene (exon 1) using PCR-RFLP method with TaqI restriction enzyme (T*CGA). DNA samples SO bulls cows (...

PURPOSE Natural killer/T-cell (NK/T-cell) lymphoma is a highly aggressive tumor for which no serological tumor marker has yet been established to be useful clinically. We investigated the potential of circulating EBV DNA as a tumor marker for this malignancy. EXPERIMENTAL DESIGN A real-time quantitative PCR assay was used to measure circulating EBV DNA. RESULTS Plasma EBV DNA levels were me...

BACKGROUND AND OBJECTIVES DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. MATERIALS AND METHODS Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce a...

Genetic diversity as an important marker of the ecological status of aquatic ecosystems is considered a unique and powerful tool to evaluate biological communities. In order to evaluate the genetic diversity among golden mullet species (Liza aurata) in the southeast and southwest coasts of the Caspian Sea by D-Loop gene sequencing, a total of 23 fin specimens of golden mullet were collected fro...