many recent studies have shown that glycosylation patterns of agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. to achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. to isolate this promoter, pcr with specific primers was perform...

The analysis of DNA sequence polymorphisms and SNPs (single nucleotide polymorphisms) can provide insights into the evolutionary forces acting on populations and species. Available population-genetic methods, and particularly those based on the coalescent theory, have become the primary framework to analyze such DNA polymorphism data. Here, I explain some essential analytical methods for interp...

Analysis of DNA sequences has greatly aided our understanding of molecular biology and genetics. Nucleotide sequences have provided a wealth of information about gene structure and function. In addition to revealing the primary structure of genes, sequencing has uncovered the phenomena of overlapping genes, segmented genes, pseudogenes and a different genetic code for mitochondria. To date over...

Conventional DNA sequencing is based on gel electrophoretic separation of the sequencing products. Gel casting and electrophoresis are the time limiting steps, and the gel separation is occasionally imperfect due to aberrant mobility of certain fragments, leading to erroneous sequence determination. Furthermore, illegitimately terminated products frequently cannot be distinguished from correctl...

We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid. Ordered sets of subclones of predetermined overlap are are generated. These can be sequenced dir...

Bioinformatics is the combination of biology and information technology. It is the branch of science that deals with the computer-based analysis of large biological data sets. Bioinformatics incorporates the development of databases to store and search data, and of statistical tools and algorithms to analyze and determine relationships between biological data sets. In this paper we have studied...

The decline of ornamental fish populations in their native habitats due to over-fishing has brought about the development of ornamental fish aquaculture, to which fish of the Anabantoidei suborder are important contributors. The genetic variations among species of this suborder were examined by mitochondrial gene sequencing analysis using the cytochrome b and 12S genes. According to the cytochr...

This paper presents a computer system for analyzing and annotating large-scale genomic sequences. The core of the system is a multiple-gene structure identi cation program, which predicts the most \probable" gene structures based on the given evidence, including pattern recognition, EST and protein homology information. A graphics-based user interface provides an environment which allows the us...