The most widespread and harmful disease of barley is net form blotch caused by the ascomycete Pyrenophora teres f. Drechsler (Ptt). A cost effective environmentally sustainable strategy for protection against Ptt to develop cultivars possessing genetic resistance. In previous GWA analysis, we identified SNP-markers associated with a resistance locus on chromosome 3H in interval 45.82–54.53 cM. ...

Knowledge about the efficiency of generations for estimating marker-associated QTLs is needed for selection. The objective of this paper is to develop a theory to compare the efficiency of segregating generations and testcrosses from the cross of two inbred lines differing in value for a quantitative trait (P1 x P2) for estimating additive, dominance and heterotic effects of QTLs by stepwise re...

Quantitative trait loci (QTL) mapping is a powerful tool for investigating the genetic basis of natural variation. QTL can be mapped using a number of different population designs, but recombinant inbred lines (RILs) are among the most effective. Unfortunately, homozygous RIL populations are time consuming to construct, typically requiring at least six generations of selfing starting from a het...

Doubled haploid (DH) technology is a powerful tool in plant breeding to reduce the time and costs needed to produce pure lines, the cornerstone of hybrid seed production. This biotechnological alternative to classic methods allows for a reduction of the typical 7–8 inbreeding generations needed to fix a hybrid genotype to only one in vitro generation. It is therefore much faster and cheaper, be...

Abstract The endogenous level of cytokinins (CKs) and auxins (Auxs) was analyzed in isolated microspores ovaries two doubled haploid (DH) lines triticale (× Triticosecale Wittm.) to better understand the mechanism microspore embryogenesis (ME)—the most efficient widely applied method producing DHs. responsiveness studied ME significantly varied. induced by pre-treating tillers with low temperat...

Autumn-seeded winter cereals acquire tolerance to freezing temperatures and become vernalized by exposure to low temperature (LT). The level of accumulated LT tolerance depends on the cold acclimation rate and factors controlling timing of floral transition at the shoot apical meristem. In this study, genomic loci controlling the floral transition time were mapped in a winter wheat (T. aestivum...