ناآ دقل رهوج ،ةيفخ ةفصب تامولعملا لاسرإ و لاصتلاا عوضوم مدقلا ذنم مامتهلاا و ديعب نمز ذنم عساو لكشب مدختست ةيصنلا قئاثولا تناآ ، صوصنلا يف تامولعملا ءافخلإ ةفلتخم اًقرط مويلا ىتح نمز ذنم دهشن نحن كلذل ) Text Steganography .( لاقملا اذه نإ ط لوانتي ةيبرعلا و ةيسرافلا صوصنلا يف تامولعملا ءافخلإ ةديدج ةقير . و دوآ ينويلا ةفصاوم يف دجوي ) Unicode ( ءايلا فرحل نافلتخم نازمر » ى « ، فاكلا فرحل كلذآو...

BACKGROUND & OBJECTIVES The difficulties in diagnosis of neonatal sepsis are due to varied clinical presentation, low sensitivity of blood culture which is considered the gold standard and empirical antibiotic usage affecting the outcome of results. Though polymerase chain reaction (PCR) based detection of bacterial 16S rRNA gene has been reported earlier, this does not provide identification o...

Dermatomycoses are very common infections caused mainly by dermatophytes. Scytalidiosis is a differential mycological diagnosis, especially in tropical and subtropical areas. Since a culture-based diagnosis takes 2 to 3 weeks, we set up a PCR-restriction fragment length polymorphism (RFLP) method for rapid discrimination of these fungi in clinical samples. The hypervariable V4 domain of the sma...

Burkholderia cepacia complex (BCC) is being increasingly recognized as an important pathogen of humans. During the year 2007-8, 39 putative BCC isolates were obtained from 21 cases and subjected to recA PCR RFLP. Twenty-four isolates were confirmed as Burkholderia cenocepacia IIIA (nineteen isolates, recA PCR RFLP type G and five isolates, recA PCR RFLP type I), six were confirmed as B. cepacia...

Cutaneous leishmaniasis (CL), a public health problem in Tunisia, is associated to three species: Leishmania (L.) infantum, L. major and L. killicki. Accurate and sensitive procedures for the diagnostic of Leishmania infection and for species identification are required to enable adequate treatment and appropriate control measures. Several PCR-methods are applied for the diagnosis and the ident...

We examined the usefulness of PCR-based restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR combined with a newly devised rapid biochemical test using microplates for identifying weakly beta-hemolytic intestinal spirochetes (WBHIS) isolated from pigs. WBHIS strains showing atypical biochemical characteristics were decisively identified at the species level by PCR-RFLP a...

Background: Cryptosporidium parvum is major protozoan parasite of both animals and humans with zoonotic significance. There a paucity information on its occurrence associated risk factors in North Eastern states, India. The present study aimed at the molecular characterization from cattle handlers West district Tripura associates analysis. Methods: Faecal samples were randomly collected (100 ea...

Mycobacterium tuberculosis sputum isolates from 38 patients, obtained in the first 6 months of 1997 in Havana, Cuba, were characterized by IS6110 restriction fragment length polymorphism (RFLP) analysis and the double-repetitive-element PCR (DRE-PCR) method. Among 41 strains from 38 patients, 24 and 25 unique patterns, and 5 and 4 cluster patterns, were found by the RFLP and DRE-PCR methods, re...