Langerhans cells (LC) are skin-resident antigen-presenting that regulate immune responses to epithelial commensal and pathogenic microorganisms. Infection of skin by human papillomavirus (HPV) is commonly persistant can promote malignant transformation. As LCs considered important in control HPV infection, we compared the transcriptome from transformed oncogenic E7 protein HPV16 healthy skin. W...

The major causative agent of cervical cancer is human papilloma virus (HPV); the viral proteins E6 and E7 induce carcinogenesis through the inactivation of the host tumor-suppressor gene. Therefore, the stable expression of specific inhibitors of E6 and E7 in cancer cells is expected to provide effective treatment for cervical cancer without affecting normal tissue. In this study, we propose a ...

Expression of HPV E5, E6 and E7 oncogenes are likely to overcome the regulation of cell proliferation and to escape immunological control, allowing uncontrolled growth and providing the potential for malignant transformation. Thus, their three oncogenic products may represent ideal target antigens for immunotherapeutic strategies. In previous attempts, we demonstrated that genetic vaccines agai...

Acute expression of E7 oncogene from human papillomavirus (HPV) 16 or HPV18 is sufficient to overcome tumor necrosis factor (TNF)-alpha cytostatic effect on primary human keratinocytes. In the present study, we investigated the molecular basis of E7-induced TNF resistance through a comparative analysis of the effect of this cytokine on the proliferation and global gene expression of normal and ...

Head and neck squamous cell carcinomas (HNSCC) is a common cancer in humans long known to be caused by tobacco and alcohol use, but now an increasing percentage of HNSCC is recognized to be caused by the same human papillomaviruses (HPV) that cause cervical and other anogenital cancers. HPV-positive HNSCCs differ remarkably from HPV-negative HNSCCs in their clinical response and molecular prope...

Human papillomavirus (HPV) infection is the most important risk factor for the development of cervical cancer. The oncogene E7 from high-risk HPV strains has the ability to immortalize epithelial cells and increase cellular transformation in culture. In this study, we explored the possibility of preventing cervical cancer growth by inhibiting HPV16 E7 expression through gene transfer of an anti...

Cervical cancer is a disease characterized by the growth of abnormal cells in cervical tissue. mostly caused Human Papillomavirus (HPV) types 16 and 18. The role genetic factors development malignancy mediated presence mutation HPV oncogene, especially oncogenes E6 E7. Oncogenic proteins E7 initiate dysregulation cellular proliferation apoptotic mechanisms targeting tumor suppressor proteins, s...

Objective In past decades, the role of high-risk HPV (HR-HPV) infection in cancer pathogenesis has been extensively studied. The viral E7 protein expressed pre-malignant cells identified as an ideal target for immunological intervention. However, cultivation vitro remains a significant challenge, well lack methods expressing and generating replication-competent recombinant particles, which pose...

As dendritic cells (DCs) are the most potent professional antigen-presenting cells, they are being tested as cancer vaccines for immunotherapy of established cancers. Although numerous studies have characterized DCs by their phenotype and function, few have identified potential molecular markers of antigen presentation prior to vaccination of host. In this study we generated pre-immature DC (pi...

Rb protein is a critical regulator of entry into the cell cycle, and loss of Rb function by deletions, mutations, or interaction with DNA viral onco proteins leads to oncogenic transformation. We have shown that the human papifloma virus (HPV)-16 E7 gene is sufficient to induce the immortalization of mammary epithelial eels (MECs). Surprisingly, the steady-state level of Rb protein in these imm...