Protein hydrolysates and peptides can show biological activities, pulsed ultrasound improves bioactivities. Among matrices from which protein be obtain, chickpea is an excellent source. The objective of this research was to evaluate the effect on globulin concentrate obtain hydrolysate (HGb) peptide fractions their bioactivity. Antioxidant activity by ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline...

A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a gamma(1)-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous p...

A study was made of the effects of 2,3-dimethylmaleic anhydride (DMA), a reagent removing positive charges, on the aggregation and surface charge of embryonic chick neural retina cells. Neural retina cells, recovered from the dissociation procedure, were cultured on a gyratory shaker and the aggregate dimaeters formed in the presence of DMA or DMA-serum dialysate, following DMA-pretreatment, or...

A method of fractionation of proteins in solution in which an electrophoretic adaptation of the principles of the Clusius column is utilized was suggested by Kirkwood in 1941 (1) and tested experimentally by Nielsen and Kirkwood (2) several years later. Recently an electrophoresis-convection apparatus of improved design has been described and successfully used in the fractionation of the pseudo...

Archibald, R. M. (1944). J. biol. Chem. 154, 643. Coulson, R. A., Ellinger, P. & Holden, M. (1944). Biochem. J. 38, 150. Ellinger, P. (1946a). Biochem. J. 40, xxxi. Ellinger, P. (1946b). Biochem. J. 40, xxxii. Ellinger, P. & Coulson, R. A. (1944). Biochem. J. 38, 265. Ellinger, P., Fraenkel, G. & Abdel Kader, M. M. (1947). Biochem. J. 41, 559. Johnson, B. C. (1945). J. biol. Chem. 159, 227. Kon...

The total protein, albumin, globulin, and immunoglobulin levels of sera from 96 children with homozygous sickle cell disease were studied. A comparison of the results with the levels found in a control group of normal children of the same age shows that the sicklers have higher total protein, globulin, and IgM levels. The amounts of albumin and IgA seen were almost the same in both groups. The ...