BACKGROUND Haemophilus influenzae type b (Hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. Enzyme-linked immunosorbent assay (ELISA) technique is the most used method to detect antibodies against H. influenzae. Available commercial ELISA kits are expensive and not always readily available, particularly for epidemiological studies. OBJECTIVES T...

An enzyme-linked immunosorbent assay (ELISA) is described for detection of anti-capsular antibodies against Haemophilus influenzae type b. Polyribosephosphate was covalently bonded to poly-L-lysine before adsorption to microtiter plates. ELISA immunoglobulin G and immunoglobulin M anti-polyribosephosphate antibody titers were comparable to total anti-polyribosephosphate antibody concentration d...

The microplate method of an ELISA was modified for identification of human blood meals from 5,325 engorged mosquitoes belonging to 12 species of Anopheles captured in 19 provinces of Iran. Four hundred and four (7.5%) specimens reacted with the ELISA anti-human alkaline phosphatase conjugate. The human blood index in nine species of Anopheles varied from 3.6 to 23.7%. The results of this field ...

Background: The current study was conducted to compare ELISA with thin layer chromatography (TLC) methods for diagnosis of morphine in the urine. Methods: Positive urine samples for morphine confirmed by immunochromatographic strips were collected from the Imam Reza Toxicology Laboratory, Mashhad University of Medical Sciences, Mashhad, Iran in 2012 for the current study. Then, the collated ...

Background: Dirofilaria immitis is a cosmopolitan zoonotic, vector-borne parasite of carnivorous animals causing dirofilariasis in human beings. Common commercial serodiagnostic tests for canine dirofilariasis usually lead to different results in their sensitivity and specificity. The present study reports development of recombinant DgK (rDgK) antigen of D. immitis for accurate immunodiagnosis o...

The performance of a commercial immunofluorescence assay (IFA commercial), an in-house in-house) and indirect enzyme-linked immunosorbent (ELISA) were evaluated in the detection antibodies anti-C. burnetii serum Q fever patients persons without disease. For study, seropositive seronegative samples for (n = 200) from bank Instituto Adolfo Lutz Brazil used. Commercial IFA was considered this stud...

Enzyme-linked immunosorbent assay (ELISA) is a valuable technique to detect antigens in biological fluids. Horse radish peroxidase (HRP) is one of the most common enzymes used for signal amplification in ELISA. Despite new advances in technology, such as a large-scale production of recombinant enzymes and availability of new detection systems, limited research is devoted to finding alternative ...