introduction: embryonic stem (es) cells are pluripotent cells derived from the inner cell mass of blastocysts. these cells are appropriate for creation of animal models of human genetic diseases, the study of gene functions in vivo and differentiation into specific types as potential therapeutic agents for several diseases. we describe here production of transgenic chimeric mouse blastocysts by...

Bone tissue engineering has been one of the most promising areas of research, providing a potential clinical application to cure bone defects. Recently, various stem cells, including embryonic stem cells (ESCs), bone marrow-derived mesenchymal stem cells (BM-MSCs), umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs), adipose tissue-derived stem cells (ADSCs), muscle-derived stem cell...

Despite the significant advances in regenerative medicine, wound healing has remained a challenging clinical problem. Skin is the largest human organ with many vital functions; therefore, any damage to its normal structure should be treated as soon as possible. Easy access to skin stem cells has created a lot of excitement in therapeutic applications. “Cell therapy” is considered a novel method...

Induced pluripotent stem cells (iPSCs) are reprogrammed from somatic cells through numerous transcription factors. Human induced pluripotent stem cell approaches are developing as a hopeful strategy to improve our knowledge of genetic association studies and the underlying molecular mechanisms.  Rapid progression in stem cell therapy and cell reprogramming provides compelling reasons for its fe...

introduction: embryonic stem (es) cells are pluripotent cells derived from inner cell mass of blastocyst. these cells can product any cell by spontaneous or directed differentiation. this study was started to produce neuron cells by directed differentiation of new murine es cell line in vitro. materials and methods: murine es cells (royan b1 derived from c57bl/6 strain) were used for in vitro n...

Background:Differentiation of Embryonic Stem Cells into Oocyte-like cells in vitro is challenging. Successful derivation of oocyte from stem cells can provide an alternative source for curing ovogenesis problems. The current study aims to demonstrate a new protocol with two different types of media for differentiating embryonic stem cells (ESCs) into oocyte-like cells ...

two basic and clinical researches accomplished during the recent years on embryonic and adult stem cells constituted a mutation in regenerative therapy. these cells can be used for treating some degenerative diseases. between them, age-related functional defects, hematopoietic and immune system disorders, heart failures, chronic liver injuries, diabetes, parkinson’s and alzheimer’s diseases, ar...

received: 23/sep/2008, accepted: 23/nov/2008oct-4/ pou5f1/ oct-3, a pou domain family protein acts as a crucial transcription factor dur- ing embryonic development. it helps in maintenance of self renewal as well as pluripotential state of embryonic stem (es) cells. its expression starts right from 2 cell stage especially prior to 8 cell stage till the blastocyst stage where it is strongly exp...