Prior treatment of mice with poly I:C or NDV enhanced the lethal effect of E. coli or B. dermatitidis endotoxins.

The experiments reported here demonstrate that under certain conditions endotoxin can interact with lysosomes in vitro. After incubation of large granular fraction with (51)Cr-labeled antigen under the conditions required for acid hydrolytic activity, radioactivity was associated with the pellet after centrifugation. This effect can be inhibited by preincubation of the large granular fraction w...

The present study deals with some new biotechnological forms of nutritional supplements and their effectiveness in helping people gut disbiosis. We have developed four different to prevent treat gastrointestinal diseases. general characteristic each supplement is given. Nutritional 1 contains modified sorbents neutralize bacterial endotoxins. 2 includes live microencapsulated Bifidobacterium La...

Bacterial endotoxins in vitro are capable of shortening the coagulation time of normal whole blood, native platelet-rich and platelet-poor plasma, and the blood of a hemophilic patient in silicone but not in glass. The point in the coagulation system at which the endotoxins act has not been found but the search has been narrowed by the demonstration that these materials act independently of leu...

A comparison of the immunogenicity of sodium deoxycholate-disrupted lipopolysaccharides from Escherichia coli cell walls revealed that these fragments, which are nonimmunogenic in the rabbit, have some activity in the mouse. This relationship was independent of the route of immunization and sex, but in both species immunogenicity was restored by dilution or dialysis. Adsorption of disrupted lip...

PURPOSE To measure the endotoxin concentration (EC) of 25 commercially available, hyaluronic acid- and hydroxypropylmethylcellulose-based (HPMC) ophthalmic viscosurgical devices (OVDs). METHODS The in vitro Limulus amebocyte lysate (LAL) assay, which indicates the presence of endotoxins originating from gram-negative bacteria, was used to determine the EC. The procedure was performed accordin...

Many steps in the analysis of rough and semirough endotoxins were found to be facilitated by the use of isobutyric acid-ammonium hydroxide solvent.