NMR studies of the internal family 2b carbohydrate binding module (CBM2b-1) of Cellulomonas fimi xylanase 11A have identified six polar residues and two aromatic residues that interact with its target ligand, xylan. To investigate the importance of the various interactions, free energy and enthalpy changes have been measured for the binding of xylan to native and mutant forms of CBM2b-1. The da...

Rational drug design is predicated on knowledge of the three-dimensional structure of the protein-ligand complex and the thermodynamics of ligand binding. Despite the fundamental importance of both enthalpy and entropy in driving ligand binding, the role of conformational entropy is rarely addressed in drug design. In this work, we have probed the conformational entropy and its relative contrib...

Results: A total of 247 plastic surgeons were included. 78.6% cited previous work at least once (mean 34.3 +/87.5 self-citations). These self-citations accounted for 4.8% of all citations. Including self-citations in bibliometric analyses increased h-indices from 9.5 +/6.9 to 10.2 +/7.6. A minority of researchers (20.1%) increased their h-index via self-citation by at least one integer value (r...

Thermodynamics of the binding of antibiotic streptomycin to bovine serum albumin have been studied using isothermal titration calorimetry in combination with fluorescence, UV–vis and circular dichroism spectroscopies. The values of van’t Hoff enthalpy calculated from the temperature dependence of the binding constant do not agree with the calorimetric enthalpies indicating temperature dependent...

Two high precision techniques, titration microcalorimetry and thin-layer optical binding measurements, have made possible the evaluation of enthalpy changes for the overall oxygenation reactions for human hemoglobin (HbAo). Although the heat of adding three oxygen molecules could not be evaluated due to the indeterminate contribution of this species to the oxygen binding curve of the protein (G...

Ion exchange chromatography is a widely used method for purification of all types biomolecules in current biotechnological downstream processes. Knowledge on the binding behavior proteins provides valuable insight understanding molecular mechanisms protein interactions biological context. However, thermodynamic parameters such as enthalpy and entropy changes that characterize adsorption are sti...

Binding of 4-methylumbelliferyl-α-D-galactopyranoside (MeUmbαGal) and the corresponding β-anomer (MeUmbβGal) to the Momordica charantia (bitter gourd) lectin (MCL) has been investigated by fluorescence spectroscopy. Binding of MeUmbαGal to MCL resulted in a decrease in the fluorescence intensity of the ligand. Saturation binding at 25°C resulted in a 17.8% decrease in the fluorescence intensity...

interaction between dna and histone h, was investigated in the presence and absence of sodium-n-dodecyl sulphate (sds) and dodecyl trimethylammonium bromide (dtab) at temperatures of 27 and 37?c, in 2.5 mm phosphate buffer, ph 6.4 by uv spectrophotometry, equilibriumdialysis and titration. the presence of 1.33 mm sds caused histone h to fold and to further contact dna. binding data were used in...