UNLABELLED BACKGROUND Biosensors have attracted increasing attention as reliable analytical instruments in in situ monitoring of public health and environmental pollution. For enzyme-based biosensors, the stabilization of enzymatic activity on the biological recognition element is of great importance. It is generally acknowledged that an effective immobilization technique is a key step to ac...

Surface immobilization of biomolecules is a fundamental step in several experimental techniques such as surface plasmon resonance analysis and microarrays. Oxime ligation allows reaching chemoselective protein immobilization with the retention of native-like conformation by proteins. Beside the need for chemoselective ligation of molecules to surface/particle, equally important is the controlle...

Porous copolymer was prepared by suspension polymerization method of styrene, maleic anhydride and divinylbenzene in a batch type reactor. The characteristics of the copolymer were evaluated using Fourier Transform IR spectroscopy and scanning electron microscopy. The glucose oxidase (GOD) enzyme was selected as a model enzyme to assess its potential applicability for immobilization purpose. Th...

Enzymatic biofuel cells have many great usages as a small power source for medical and environmental applications. In this paper, we employed carboxylated multiwall carbon nanotube- (1-ethyl-3-methylimidazolium bis (trifluoromethyl sulfonyl) imide) ionic liquid nanocomposite on two different electrodes (glassy carbon and carbon felt) for immobilizing alcohol dehydrogenase. The properties of the...

α-Nitrophenyl derivatives of glycosides are convenient substrates used to detect and characterize α-N-acetylgalactosaminidase. A new procedure combining chemical biocatalytic steps was developed prepare 4-nitrophenyl-2-acetamido-2-deoxy-α-D-galactopyranoside (4NP-α-GalNAc). The α-anomer prepared through synthesis an anomeric mixture followed by selective removal the β-anomer using specific enzy...

glucose, as the major energy source in cellular metabolism, plays an important role in the natural growth of cells. herein, a simple, rapid and low-cost method for the glucose determination by utilizing glucose oxidase and cdte/thioglycolic acid (tga) quantum dots (qds) on a thin layer chromatography (tlc) plate has been described. the detection was based on the combination of the glucose enzym...

The concurrent effects of two enzyme inhibitors have been analysed previously with the Yonetani-Theorell plot to obtain insight into the interactions between bound inhibitors. This procedure, like many other traditional graphical methods in enzymology, is based on the estimation of intersecting tendencies in a family of lines. In a recent paper from this laboratory [Chan (1995) Biochem. J. 311,...

The halogenases are a group of enzymes that have only come to the fore over the last 10 years thanks to the discovery and characterization of several novel representatives. They have revealed the fascinating variety of distinct chemical mechanisms that nature utilizes to activate halogens and introduce them into organic substrates. Computational studies using a range of approaches have already ...

Now, more than ever, enzymology and its development can be considered of vital importance to the progression of the biological sciences. With an increase in the numbers of enzymes being identified from genomic studies, enzymology is key to defining the structural and functional properties of these enzymes in order to establish their mechanisms of action and how they fit into metabolic networks....

The present study aimed to the batch production of CGTase (cyclomaltodextrin-glucanotransferase) with Bacillus firmus strain 37 free and immobilized in bovine bone charcoal mode a fluidized bed reactor, respectively. is an innovative support material for immobilization microorganisms’ producers enzymes use this microbial allows its reuse significant cost reduction process. fermentation cells wa...