materials and methods the expression of hyaluronidase antigenic fragments was optimized using iptg concentration, time of induction, temperature, culture, and absorbance of 0.6-0.8-1 at 600 nm. afterwards, the expressed proteins were purified and the enzymatic activity was assessed by turbid metric method. results data indicated that maximum protein is produced in od = 0.8, 0.5 mm isopropyl β-d...

thermoplastic starch (tps) materials present several advantages to the plastic industry andwhen blended with other materials they can exhibit improved mechanical and moisturesensitivity properties compared to pure tps materials. further investigations on tps: pvablends are of particular interest due to their excellent compatibility and improved properties suchas tensile strength, elongation, to...

enzymatic extract preparation from pseudomonas aeruginosa km110 under accession no. hq730879 with lipase activity (0.3 u/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/l total fat content. the pretreatment was optimized for 48 h hydrolysis time, at 45æ% c with 10% v/v enzymatic extract. the biological treatment of synthetic dairy wastewat...

This study was aimed for the investigation of effect pretreatment procedure alkaline, based on chemical arrangement, surface morphology, structural composition and enzymatic assimilation sugarcane bagasse sugars ethanol production. Alkali (0 to 8% w/v NaOH) assists reduce lignin portion (from 19.57±0.03% 9.91±0.02%) increase cellulose content treated SB 34.66±0.05% 63.58±0.05%) simultaneously. ...

The correct assignment of amide groups to the glutamyl and aspartyl residues determined from analyses of acid hydrolysates continues to be an important problem in the delineation of protein and peptide structure. This problem is illustrated by recent discussions by Potts et al. (1) and Smyth, Stein, and Moore (2) on the elucidation of ribonuclease structure and by Chibnall et al. (3) on the est...

background  isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...

A semiautomated, enzymatic, fluorometric method for the determination of the low glucose concentrations normally present in urine from subjects in the fasting state is described. The method is developed from a manual, enzymatic, fluorometric technic for the determination of urinary glucose using hexokinase (HK) and glucose-6phosphate dehydrogenase (G-6-PDH). The procedure is based on measuremen...

A fully enzymatic method for measurement of l(+)-lactate has been adapted for use with a centrifugal analyzer. The procedure is based on a two-point fixed-time kinetic approach for elimination of static interferences, and commercially available reagents are used. Specimen collection and preservation are simplified by the use of standard evacuated tubes containing sodium fluoride. Under these co...

Bioactive peptides showing Angiotensin-I-Converting Enzyme inhibitory (ACEi) activity can control blood pressure. They be produced from various protein sources, including fish gelatin. This article provides a systematic review and meta-analysis on gelatin extraction, conditions of enzymatic hydrolysis, characteristics ACEi peptides, effect hydrolysis procedure peptides. Fish preparation uses so...