the microplate method of e nzyme-l i nke d i rnmuno s o r be n t a s s ay (elisa) , usi ng alkaline phosphatase anti-human i gg conj ugate , was modified and appl ied in t he dete c t i on of the human b lood specimens among the blood samples prep a r ed fr om human and laborat or y anima l s i n t he form of small dr ied b loodst a i ns on f ilt e r paper . the modi f i e d elisa t e chnique w...

There are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals EDCs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (VTG) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs especially...

An indirect enzyme-linked immunosorbent assay (ELISA) and a dot enzyme assay (DEA) were used to determine the specificities of Cryptococcus neoformans factor sera to serotype type-specific capsular polysaccharides, glucuronoxylomannans (GXMs). Pure and chemically characterized GXMs were obtained from representative isolates of C. neoformans serotypes A, B, C, and D. Distinctive specificity patt...

Enzyme-linked immunosorbent assay (ELISA) has been served as both the workhorse and gold standard in immunoassay for its high specificity, sensitivity, accuracy, despite known shortcomings limitations....

This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully a...

Gold nanoparticle-based diagnostic methods have attracted much attention due to their simplicity, high sensitivity and low cost. These methods are mostly used for early and efficient detection of various pathogenic factors and assessment of gene expression and nanoparticles stability. In this study, melon plants were cultured in vivo and then, RNA was extracted from leaf explants. A 40 bp probe...

A simple, reliable, and universal method is demanded for routine determination of carbonic anhydrase (CA) activity, overcoming the limitations previous assays that are inaccurate, complicated, expensive, or limited to a specific enzyme family. The most widely used Wilbur–Anderson assay was modified improve speed, accuracy, precision by employing temperature controllable UV/Vis spectrophotometer...