DNA ligase has been purified to near-homogeneity from the extract of bovine thymus with a yield of 5%. The purified enzyme catalyzed the joining of single-stranded breaks in duplex DNA at a rate of 33 nmol of phosphodiester bonds/min/mg of protein. The purified enzyme was homogeneous as judged by polyacrylamide gel electrophoresis and Ouchterlony double diffusion analysis. The enzyme is compose...

To avoid the secondary pollution that inorganic corrosion inhibitor may cause in circulating cooling water, the bio-corrosion inhibition effect of lysozyme, catalase, lipase, and laccase, as the biologic inhibitors without the above problem, and the increased bio-corrosion inhibition effect of Ca+2 and Mg+2 are studied. An enzyme with corrosion properties was selected by rotary coupon test. The...

• Enzyme types and their sources affect enzymatic in resistant pollutants remediation. Enzymatic bioprocesses demonstrate priority wastewater treatment. Enzymes show promise resisting removed from wastewater. Identification ecotoxicity assay of byproducts are important. mediators improve processes but there drawbacks. Resistant like oil, grease, pharmaceuticals, pesticides, plastics difficult t...

Alanine scanning mutagenesis of a recently reported prostate cancer cell-selective Protein Transduction Domain (PTD) was used to assess the specific contribution each residue plays in cell uptake efficiency and cell-selectivity. These studies resulted in the identification of two key residues. Extensive mutagenesis at these key residues generated multiple mutants with significantly improved upt...

Abstract The regulation of the activity and selectivity phospholipase A2 (PLA2), which is capable cleaving fatty acid in second position ( sn-2 ) phospholipid, carried out through membrane-binding catalytic sites enzyme. For hydrolytic activity, PLA2 must first bind to phospholipid membrane, binding efficiency depends on composition membrane. site formed by several tens amino acids its differs ...

Abstract Within cells, the close spatial arrangement of cascade enzymes facilitates channeling intermediates and enhances reaction efficiency. Reconfigurable DNA nanostructures, owing to their structural controllability precise addressability, are promising tools for mimicking such processes. In this study, a 3D origami scaffold, with dynamic shape transformation from its open boat form closed ...

Co-precipitation of enzymes in metal-organic frameworks is a unique enzyme-immobilization strategy but challenged by weak acid-base stability. To overcome this drawback, we discovered that Ca2+ can co-precipitate with carboxylate ligands and under ambient aqueous conditions form [email protected] material composites stable wide range pHs (3.7–9.5). We proved on four varied isoelectric points, m...

Catfish (Clarias gariepinus) is a freshwater fish that widely cultivated in Indonesia. Production cost of farming activities spend 60-70% on the use feed. Therefore, feeding must be effective and efficient so catfish can make good it for its growth. The addition an exogenous enzyme form papain increase efficiency feed utilization. purpose this study was to determine effect growth survival sangk...