Background Trypsin is an enzyme widely used for digestion of peptides in proteomic research. However, this enzyme is one of the least stable of the neutral proteases and presents rapid autolysis, which causes a decrease in catalytic efficiency and a consequential increase in costs of biotechnological process. Immobilization of the enzyme offers the advantages of enhanced stability, protection a...

Among the many biological entities employed in development of biosensors, enzymes have attracted most attention. Nanotechnology has been fostering excellent prospects enzymatic since enzyme immobilization onto conductive nanostructures can improve characteristics that are crucial biosensor transduction, such as surface-to-volume ratio, signal response, selectivity, sensitivity, conductivity, an...

Background: Immobilization is an approach in industry to improve stability and reusability of urease. The efficiency of this technique depends on the type of membrane and the method of stabilization. Methods: The PEI-modified egg shell membrane was used to immobilize urease by absorption and glutaraldehyde cross-linking methods. The membranes were characterized by Fourier-transform infrared sp...