an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...

Pelargonium graveolens (Geranium) is a source of the finest quality fragrance and its essential oils are used as antibacterial, antifungal agents. The aim current research to determine chemical constituents in oil P. by GC-MS evaluate antidiabetic activity via α-glucosidase inhibition assay. composition was determined GC/MS assessed through enzyme in-vitro models. analysis determines 36 compone...

Neuraminidase inhibitor assay requiring complicated step-by-step operation was successfully integrated into ”Single-Step” by using CPC sensor. We developed a combinable PDMS capillary (CPC) sensor for enzyme inhibition assay using the concave and convex type PDMS substrates. In the conventional enzyme inhibitor assay, complicated analytical procedures, such as mixing and dissolution, are requir...

Varicose vein is one of the common vascular diseases, in which veins become widened, bulged and twisted. Development drug to combat varicose a challenging task due complex nature disease non-availability specific animal model system. In this context, relevant experimental models were developed polyherbal formulations HVVL-041702 liniment intended for topical use (200 µl/ animal) HVVT-041701 gra...

objective(s):one strategy for the treatment of diabetes is inhibition of pancreatic α- amylase. plants contains different chemical constituents with potential for inhibition of α-amylase and hence maybe used as therapeutic. materials and methods: urtica dioica and juglans regia linn were tested for α-amylase inhibition. different concentrations of leaf aqueous extracts were incubated with enzym...

A new series of N-Mannich bases 2-Phenyl-5-benzimidazole sulfonic acid have been synthesized through amino methylation reaction with secondary amines. The two moieties were held together a methylene bridge, which comes from formaldehyde (Formalin Solution 37%) used in the reaction. Chemical structures newly compounds confirmed using FT-IR, 1HNMR and 13CNMR. Different vitro assays including Anti...

A series of polysubstituted 1,4-dihydropyridines (4a-i) were designed and developed using Ph-B(OH)2 catalyst. Further, compounds confirmed by various spectroscopic techniques. After that all studied for molecular docking against the human peroxidase enzyme (1PRX). Results revealed many exhibited low binding score. To get a reference from study, synthesized molecules evaluated in vitro antioxida...

This study analyzed the inhibition activity of Swietenia mahagoni seeds extract on α-glucosidase and α-amylase enzymes inhibition assays.  Swietenia mahagoni seeds were extracted by using Supercritical Carbon Dioxide (SC-CO2) extraction at pressures of 20- 30 MPa and temperatures of 40- 60°C.  The oil yields obtained were analyzed with α- glucosidase and α- amylase enzymes inhibition assays.  A...

Derivatives of phenyl-keto butenoic acids have been reported to be inhibitors of pyruvate decarboxylase, (PDC). The inhibition of transketolase, a thiamine requiring enzyme such as PDF, by meta nitrophenyl derivative of 2-oxo-3-butenoic acid (MNPB) is reported here. These studies indicate that the inhibitor binds to the enzyme at the active site. A two-step inhibition was observed, first th...