background several studies have been carried out to evaluate the effects of dialysis on o2 saturation. while the dialysis procedure may lead to hypoxia under different circumstances, there are few studies available on the effects of membrane type on o2 saturation in these patients. objectives this study was to appraise the effects of high and low flux membrane on pulse oxymetery in dialysis pat...

Intact secretory granules isolated from bovine adrenal medulla express tyrosine hydroxylase (TH) activity. Granule-associated TH sediments on continuous sucrose gradients with dopamine beta-hydroxylase, a marker for granule membranes, indicating that TH is associated with chromaffin granules. Membranes prepared from lysed granules retain TH, whereas granule contents are free of the enzyme. TH i...

We have found convincing evidence for the presence of Na-K-ATPase and high potassium (K) and low sodium (Na) concentrations in the erythrocytes of some dogs associated with hereditary high concentrations of erythrocyte glutathione and some amino acids. glutamate. glutamine. and aspartate. The Na-K-ATPase activity of the erythrocyte membranes of the dogs was about 3 times that of human erythrocy...

When photosynthetic membranes from Rhodospirillum rubrum, devoid of loosely bound small molecules and proteins, were passed through a French-pressure cell, the enzyme adenosine-5'-triphosphatase (EC 3.6.1.3.) (ATPase) was released into the soluble fraction. The solubilized ATPase was purified to homogeneity. In many respects it behaved like the enzyme purified by other workers, but it also hydr...

alkaline phosphatase closely resembled that extracted from microsomes of human liver in some physicochemical respects. The specific activities of these enzymes in the complex were found to be of similar order of magnitude as compared with those of the same enzymes in plasma membranes isolated from the human liver. From the precipitate formed by incubating the complex with its antibody, all the ...

We labeled IgG with phospholipase C, using 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide. Enzymaticactivity of the resulting conjugate was inhibited when it was complexed with human IgG, but rabbit or goat IgG was not effective in suppressing the enzyme activity. Normal erythrocytes were used as substrate for the enzyme, enzymatic activity being assessed by measuring the release of hemoglobin...

By JAMES G. WHITE AND WILLIAM Knivrr T lIE UTILIZATION of adenosine triphosphate ( ATP) by human platelets during clot formation and retraction is important to the functioning of these cells in clotting physiology.’ 2 Recent developments in histochemical and electron microscopic methods have permitted identification of enzymes hydrolyzing ATP at the fine structural level.t We have applied these...

We have found convincing evidence for the presence of Na-K-ATPase and high potassium (K) and low sodium (Na) concentrations in the erythrocytes of some dogs associated with hereditary high concentrations of erythrocyte glutathione and some amino acids. glutamate. glutamine. and aspartate. The Na-K-ATPase activity of the erythrocyte membranes of the dogs was about 3 times that of human erythrocy...