Among the many biological entities employed in development of biosensors, enzymes have attracted most attention. Nanotechnology has been fostering excellent prospects enzymatic since enzyme immobilization onto conductive nanostructures can improve characteristics that are crucial biosensor transduction, such as surface-to-volume ratio, signal response, selectivity, sensitivity, conductivity, an...

This study aims to design an alternative way for the laminated fabric manufacturing with a cleaner production method, by using bio-based coating formulation and time/cost saving, environmentally friendly UV-curing technology, compared conventional petroleum based, heat solvent requiring laminating process. For this purpose, acrylated epoxidized soybean oil oligomer was synthesized included in f...

The aim of this study was to find the optimal conditions of a newly isolated Streptomyces strain JRS 19. Screening for keratinase producing bacteria and their keratinase production were investigated. The keratinase produced by the isolate was purified by chromatographic techniques and the optimum pH maintained was 7.0 and maintained at different temperatures Viz., 20ºC, 25ºC, 30ºC, 35ºC and 40º...

We discuss, from an industrial point of view, the scope and possibilities of recombinant DNA technology for "diagnostic enzyme" production and application. We describe the construction of enzyme-overproducing strains and show how to simplify downstream processing, increase product quality and process profitability, improve diagnostic enzyme properties, and adjust enzymes to harsh assay conditio...

The TRM1 gene of Saccharomyces cerevisiae encodes a tRNA modification enzyme, N2,N2-dimethylguanosine-specific tRNA methyltransferase, which modifies both mitochondrial and cytoplasmic tRNAs. The enzyme is targeted to mitochondria for the modification of mitochondrial tRNAs. Cellular fractionation and indirect immunofluorescence studies reported here demonstrate that this enzyme is also localiz...

objective(s): enzyme immobilization via nanoparticles is perfectly compatible against the other chemical or biological approximate to improve enzyme functions and stability. in this study lactoperoxidase was immobilized onto silica-coated magnetite nanoparticles to improve enzyme properties in the presence of cadmium chloride as an inhibitor. materials and methods:  the process consists of the ...

Thattekad bird sanctuary, located in the Western Ghats of Kerala, India, which hosts an unexplored microbial community, is selected for present investigation. Microbes play a major role mineral recycling and nutrient absorption by flora fauna habitat. Various bacterial extracellular enzymes facilitate all these activities. The increasing demand favor green technology encouraged us to focus on e...

As a safeguard against oxidative stress, the balance between the main antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) was believed to be more important than any single one, for example, dual-functional SOD/CAT enzyme has been proved to have better antioxidant ability than either single enzyme. By combining traditional fusion protein tec...

nanoDSF, the miniaturized differential scanning fluorimetry technology, is a revolutionary method to determine the thermostability of proteins by following changes in their intrinsic fluorescence. In this comparative study, the Prometheus NT.48 was used to determine the thermal stability of the membrane esterase PA2949 from Pseudomonas aeruginosa in presence of various detergents. The detergent...