Comparisons were made between enzymic indices of myocardial infarct size (total creatine kinase appearance and peak enzyme activity) measured during the acute state of a first myocardial infarct in 32 male patients, and analysis of contraction abnormalities in biplane left ventricular cineangiocardiograms performed one month later. The cineangiocardiograms were analysed independently by two rad...

The photo-oxidation of lysozyme (hen’s egg white), sensitized by methylene blue in 84% acetic acid solution, causes the selective modification of the methionyl residues, which are converted to the sulfoxide. The photo-oxidized protein shows a drastically reduced enzymic activity. The data obtained by comparing the sensitivity to proteolytic enzymes, as. well as the accessibility of disulfide bo...

Obtained from pSj5, the cDNA gene encoding GST antigen of Schistosoma japonicum (Philippine strain) was ligated with efficient temperature-dependent PBV220 vector which was constructed in CAPM, and then introduced into host bacterium-DH5 alpha (E. coli) by transformation. Transformants were selected by ampicillin and recombinant clones were identified by restriction mapping. The result showed t...

Clostridium cylindrosporum formyltetrahydrofolate synthetase tetramers cross-linked with dimethyl suberimidate remained active in the absence of the monovalent cations normally required for enzymic activity and the tetrameric conformation. The modified enzyme was analyzed by sodium dodecyl sulfate electrophoresis, sedimentation velocity, and gel permeation chromatography. Under the experimental...

calculating the extra rate in this unit at a series of instants during the course of the manometric experiment. These extra rates are then extrapolated to zero time so as to eliminate changes in the activity of the enzyme during the actual experiment owing to (i) progressive alterations in pH, substrate and buffer concentrations as C02 is taken up or evolved, (ii) destruction brought about byth...

Amylase distribution was studied in guinea pig pancreas microsomes fractionated by centrifuging, for 2 hr at 57,000 g in a linear 10 to 30% sucrose gradient, a resuspended high speed pellet obtained after treating microsomes with 0.04% deoxycholate (DOC).(1) Amylase appeared in the following positions in the gradient: (a) a light region which contained approximately 35% of total enzymic activit...

objective: the role of herbs against the free radicals have been put forth recently in combating many diseases. the aim of this study was to elucidate the in vitro capacity and in vivo antioxidant properties of sedimental extract of tinospora cordifolia (setc). materials and methods: setcwas subjected to in vitro chemical analysis such as 1,1-diphenyl-2-picrylhydrazyl (dpph), nitric oxide, hydr...

Schwartz et al. (1) have described the application of the continuous flow system of analysis, developed by Skeggs (2), for the automatic assay of enzymic activities involving the nicotinamide adenine dinucleotide cofactors [NAD (H) and NADP(H) ] .2 This paper describes a further development of this application to the continuous monitoring of column effluents. Enzymes which have activities invol...

The 3'-pyrophosphate derivative of CoA was synthesized by using the excreted 5'-to-3' pyrophosphoryl-transferring enzyme from Streptomyces adephospholyticus and ATP as donor and dephospho-CoA as acceptor. Cofactor activity of this new coenzyme A derivative was tested with Clostridium kluyveri phosphotransacetylase and hog heart succinic thiokinase. With the phosphotransacetylase, 3'-pyrophospho...