Surface sterilization is a vital step in preparation of healthy and viable explants tissue culture. Most surface contaminants can be eliminated by with suitable sterilizing agent. The study aimed to present an effective disinfection method for Clinacanthus nutans shoot regeneration using nodal segments. A total four different approaches were conducted treating various concentrations Sterilizing...

result from other techniques, such as those requiring aeration of cultures. The exhaust air from such operationls must be sterilized and the most reliable means of doing this is incineration. The 1 cfm capacity of the incinerator described above is sufficient for mnost laboratory scale operations. If provision is made for cooling the exit air, the apparatus may be employed as a source of steril...

Thc sterilizing effect of supercritical carben dioxide (SC-CO,) is described as compared with gaseous C02(G-CO,) and liquid CO,(L-CO,). Baker's yeast, ltscherichia eoli, Staphylococcus aure us and eonidia'ofAspergiilus niger were sterilized by treating with SC-CO, at 200 atm and 350C when the water contcnt of each microorganism was 70 .90%, However, dry cells with a water content of 2 .1O% coul...

Previous work in our laboratory demonstrated that passive transfer of porcine reproductive and respiratory syndrome virus (PRRSV)-neutralizing antibodies (NA) protected pregnant sows against reproductive failure and conferred sterilizing immunity in sows and offspring. We report here on the dose requirement for protection by passive transfer with NA in young weaned pigs. The presence of a 1:8 t...

Individuals living in malaria endemic areas are subject to repeated infections yet fail to develop sterilizing immunity, however, immunization of mice with attenuated sporozoites or subunit vaccines has shown the ability to protect mice against a sporozoite challenge. We recently reported that mice primed with dendritic cells coated with the dominant circumsporozoite CD8 T cell epitope from Pla...

Tests to determine the potential use of a quaternary ammonium resin-triiodide complex as a sterilizing agent showed that it was an ineffective sporicide and that bactericidal activity was impaired by complex milieu.

Ethylene oxide is widely used as a sterilizing agent for medical and food applications and products. However, due to its broad range of flammability (2.6 % to 100 %) in air, this gas presents a particularly serious explosion hazard, which is compounded by extremely stringent and ever increasing requirements for pollution control equipment. Many emission control devices contain potential ignitio...