The difference products (DP) of representational difference analyses (RDA) were used as hybridization probes on cDNA arrays. The effectivity of RDA products obtained with increasing driver/tester ratios (DP 1 = 100:1, DP 2 = 800:1 and DP 3 = 400,000:1) to isolate differentially expressed genes was compared with the effectivity of conventional differential hybridizations. Pacreatic cancer and co...

"Sense of place" offers esource managers a way to identify and respond to the emotional and spiritual bonds people form with certain spaces. We examine reasons forthe increasing interest in he concept and offer four broad recommendations for applying sense of place to ecosystem management. By i itiating a discussion about sense ofplace, managers can build a working relationship withthe public t...

Today’s rapid development of powerful tools for gene expression analysis provides unprecedented resources for elucidating complex molecular events. The objective of this work has been to apply, combine and evaluate tools for analysis of differential gene expression using atherosclerosis as a model system. First, an optimised solid-phase protocol for representational difference analysis (RDA) wa...

Helicobacter infection leads to chronic inflammation of the stomach. Although the infection persists in spite of an immune response, animal studies have shown that adjuvant-based oral vaccines can protect against infection and even eliminate established infection. These vaccines are thought to induce a Th2 immune response, counterbalancing the Th1 response seen with natural infections. As a pre...

sometimes, disharmony among the three powers of sovereignty emerges as an important crisis in political system and reduces its efficiency. in this article, analyzing the disharmony crisis, a theoretical description regarding the main indicators of this crisis has been offered. in the framework of the above mentioned theoretical description the main indicators of the disharmony crisis are 1) the...

Representational difference analysis (RDA) is a powerful technique for cloning the differences between genomes, and has recently been adapted for cloning differentially expressed genes. RDA, like other PCR-based differential screening methods, is prone to the production of false positives. We have identified a major source of false positives in RDA of cDNA and have introduced improvements which...

BACKGROUND The establishment of staff canteens is expected to improve the nutritional situation of Cambodian garment workers. The objective of this study is to assess the nutritive value of low-price model lunch sets provided at a garment factory in Phnom Penh, Cambodia. METHODS Exemplary lunch sets were served to female workers through a temporary canteen at a garment factory in Phnom Penh. ...

OBJECTIVE The aim of this study was to validate the EAR cut-point method for assessing the prevalence of nutrient inadequacy at the population level. DESIGN AND SUBJECTS Different methods for estimating the prevalence of inadequate intake were compared: the cut-off point method, with cut-off points at the Recommended Dietary Allowance (RDA), 0.66 RDA, 0.50 RDA and the Estimated Average Requir...