Estrogen is central to many physiological processes throughout the human body. We have previously shown that the G protein-coupled receptor GPR30 (also known as GPER), in addition to classical nuclear estrogen receptors (ER and ER), activates cellular signaling pathways in response to estrogen. In order to distinguish between the actions of classical estrogen receptors and GPR30, we have previo...

Postmenopausal women represent a vulnerable population towards endocrine disruptors due to hormonal deficit. We previously demonstrated that chronic exposure of ovariectomized C57Bl6/J mice fed high-fat, high-sucrose diet low-dose mixture chemicals with one dioxin, polychlorobiphenyl, phthalate, and bisphenol A triggered metabolic alterations in the liver but intestine was not explored. Yet, ga...

Radiolabeled anti-estrogen cu-[4-pyrrolidinoethoxylphenyl4-methoxy-cY’-nitrostilbene ([3HlCI-628) of high specific activity (16 Cilmmol) and radiochemical purity (>95%) has been prepared by catalytic tritium-iodine exchange and purified by alumina column chromatography. Its interaction with estrogen receptors in immature (day 20 to 24) rat uterus is studied in vitro and in ho. [3H]CI-628 (CI) c...

A nuclear subfraction containing bound estrogen receptor in presumed complex with its nuclear acceptor site has been partially purified from hen oviduct. Sucrose density gradient ultracentrifugation was used to separate mechanically sheared chromatin (i.e. lysed nuclei) into several fractions which differed in protein to DNA ratio as well as in vitro template activity. Gradient fractions were t...

Sexual maturation and differentiation of the rat brain are believed to result from the interaction of gonadal steroids with specific neural receptors during late fetal and early postnatal life. A variety of evidence indicates that the first appearance of estrogen receptors in the hypothalamus-preoptic area (HPOA) during the perinatal period is a crucial evident underlying these processes. Howev...

Estrogens regulate the formation of excitatory synaptic connections in the hippocampus of female rats. Because the adult hippocampus has a very low concentration of intracellular estrogen receptors, it is unclear whether a conventional genomic mechanism is involved. Nonsteroidal estrogen antagonists are useful tools to study estrogen action because they can provide pharmacological data in favor...

The steroid hormone estrogen regulates many functionally unrelated processes in numerous tissues. Although it is traditionally thought to control transcriptional activation through the classical nuclear estrogen receptors, it also initiates many rapid nongenomic signaling events. We found that of all G protein-coupled receptors characterized to date, GPR30 is uniquely localized to the endoplasm...

Silibinin is a natural flavonoid with anti-diabetic activity. Glucagon-like peptide−1 (GLP−1), an intestinal hormone mainly secreted from L cells, which regulates insulin production and sensitivity, appears to be potential therapeutic strategy for T2DM. The current study aims determine the protective effect of silibinin against palmitic acid (PA)-induced damage in GLUTag cells. results revealed...

We examined the relationship of a history of miscarriage to the ratio of tumor estrogen receptors to progesterone receptors in 74 women with breast carcinoma evaluated between 1988 and 1990. Only women who had been pregnant and whose tumor contained both estrogen and progesterone receptors were included. Women with breast carcinoma and a history of two to four miscarriages had significantly low...

  Background and Objectives: Presence of steroid hormone receptors (estrogen and progesterone) in the tumor tissues of various organs correlates with response to therapy and prognosis. Since their role in ovarian cancer is still controversial, in this study we investigated the expression and prognostic value of the estrogen receptor (ER) and progesterone receptors (PR) in ovarian papillary ser...