Fluorescence molecular imaging/tomography may play an important future role in preclinical research and clinical diagnostics. Time-and frequency-domain fluorescence imaging can acquire more measurement information than the continuous wave (CW) counterpart, improving the image quality of fluorescence molecular tomography. Although diffusion approximation (DA) theory has been extensively applied ...

Fluorescence measurements are easily made with the aid of a cryogenic fiber optic probe. Emission wavelength time matrixes, excitation emission matrixes, and time-resolved excitation emission matrixes are rapidly collected with a pulsed tunable dye laser, a spectrograph, and an intensified charged-coupled device. Compound identification is based on spectral and lifetime information. The potenti...

Food is a complex matrix of proteins, fats, minerals, vitamins, and other components. Various analytical methods are currently used for food testing. However, most the require sample preprocessing expensive chemicals. New needed quick economic measurement quality safety. Fluorescence spectroscopy simple method to measure quality, without preprocessing. This technique has been developed samples ...

This paper presents the development and implementation of a Dual Emission Laser Induced Fluorescence (DELIF) technique for the measurement of film thickness and temperature of tribological flows. The technique is based on a ratiometric principle that allows normalization of the fluorescence emission of one dye against the fluorescence emission of a second dye, eliminating undesirable effects of...

A quantitative and comprehensive knowledge of leaf fluorescence is required for the interpretation of fluorescence signals at the canopy level and also for the modelling of leaf and canopy fluorescence. In this work we present full range fluorescence excitation and emission spectra of intact leaves, expressed in units of apparent spectral fluorescence yield, from both the adaxial and the abaxia...

The absorption, excitation and emission spectra of methotrexate (MTX) solutions in natural saline water and sodium hydroxide at 10(-4)-10(-5) MpH=8.4 were measured, while they were exposed to coherent and uncoherent visible and near ultraviolet (UV) light. Xe and Hg lamp and also a nitrogen pulsed laser radiation were used. The absorption spectra exhibited spectral bands between 225 nm and 450 ...

The cross-linking with reducing sugars, known as glycation, is used to increase stiffness and strength of tissues and artificial collagen-based scaffolds. Nondestructive characterization methods that report on the structures within these materials could clarify the effects of glycation. For doing this nondestructive evaluation, we employed an in situ one-photon fluorescence as well as multiphot...

This paper presents the automatic discrimination of geographical origins of milks from Western Yunnan Plateau areas and eastern China by excitation-emission fluorescence spectrometry and chemometrics. Genuine plateau milks (n = 60) and milks from eastern China (n = 89) are scanned in the regions of 180-300 nm for excitation and 200-800 nm for emission. Different options of data analysis are inv...

We report on a novel acousto-optic tunable filter (AOTF)-based near-infrared (NIR) fluorescence excitation-emission matrix (EEM) spectroscopy technique for rapid multifluorophore characterization. We implement a unique light filtering module design by using cascaded AOTFs coupled with three orthogonally oriented polarizers to effectively remove the side-ripple artifacts of AOTFs as well as by u...