PURPOSE To compare two semiautomated methods of evaluating endothelial cells of eye bank corneas. METHODS Using a commercially available semiautomatic endothelial analyzer, seven observers determined the endothelial cell density (ECD), coefficient of variation (CV) of cell area, and the percentage of hexagonal cells (hexagonality) of the light microscopic images of the endothelium of 30 organ...

PURPOSE To identify differentially expressed genes in keratoconus (KC) corneal fibroblasts. METHODS Stromal keratocytes (having a fibroblast morphology) from KC keratoplasty specimens and eye bank donor corneas were isolated and expanded using a serum containing medium. RNA was isolated from three KC fibroblast cultures and five eye bank donor cornea fibroblast cultures. The targets from the ...

PURPOSE To quantify and characterize dolichol species in cataractous and clear human lenses. METHODS Whole lenses were collected from cadaver eyeballs from the C.H. Nagri Eye Bank and Red Cross Society Eye Bank (Ahmedabad, India). Cataractous nuclei were collected after extracapsular cataract extraction (ECCE). Wet weight for all the lenses was taken and were stored at -50 degrees C until use...

AIMS To comprehensively study the gross anatomy of human corneal innervation. METHODS Twenty-one specimens, including 12 normal human corneas from seven deceased patients, two eye-bank corneo-scleral buttons, two eye-bank corneo-scleral rims and five post-surgical specimens from three patients with keratoconus were studied. Corneal whole mounts were stained for cholinesterase enzyme using the...