BACKGROUND Cultures of human ovarian granulosa lutein (hGL) cells are broadly used in experimental studies. The choice of the culture medium is important for the optimization of the conditions for culture of hGL cells. AIM To compare the efficiency of a basic salt solution and eight different defined media on the culture of hGL cells. MATERIALS AND METHODS Cultures of the HGL-5 cell line we...

Article history: Received 23 October 2010 Received in revised form 4 January 2012 Accepted 6 January 2012 Available online 14 January 2012 JEL classification: F10 F12 F14 F16 L22 J24

The general properties of the balance f12 = 2∙f(O) – f(H), as linear combination elemental balances: f1 f(H) for H and f2 f(O) O, formulated electrolytic systems, are presented. These properties/regularities inherently related to (LC) with charge (f0) other elemental/core balances fk f(Yk) (Yk ≠ H, O), expressed by, where multipliers dk involved oxidation numbers (ONs) elements in system questi...

New nine-dimensional (9D), ab initio electric dipole moment surfaces (DMSs) of methane in its ground electronic state are presented. The DMSs are computed using an explicitly correlated coupled cluster CCSD(T)-F12 method in conjunction with an F12-optimized correlation consistent basis set of the TZfamily. A symmetrized molecular bond representation is used to parameterise these 9D DMSs in term...

Two new cylindrically symmetric inhomogeneous string cosmological models are investigated in presence of magnetic field. We have assumed that F12 is the only non-vanishing component of electromagnetic field tensor Fij . The Maxwell’s equations show that F12 is the function of x alone whereas the magnetic permeability μ̄ is the function of x and t both. To get the deterministic solution, it has b...

The plasma contact activation (CAS) and kallikrein/kinin (KKS) systems consist of 4 proteins: factor XII, prekallikrein, high molecular weight kininogen, and the bradykinin B2 receptor. Murine genetic deletion of factor XII (F12(-/-)), prekallikrein (Klkb1(-/-)), high molecular weight kininogen (Kgn1(-/-)) and the bradykinin B2 receptor (Bdkrb2(-/-)) yield animals protected from thrombosis. Wit...

Explicitly correlated F12 methods are becoming the first choice for high-accuracy molecular orbital calculations and can often achieve chemical accuracy with relatively small Gaussian basis sets. In most calculations, the many three- and four-electron integrals that formally appear in the theory are avoided through judicious use of resolutions of the identity (RI). However, for the intrinsic ac...

A previous study from our laboratory demonstrated the effects of in vitro three-dimensional(3D)-Printed collagen scaffolds on maintenance cryopreserved patient-derived melanoma explants. However, it remains unknown precisely whether culture condition influences 3D-printed loaded with explant(3D-PCS-PDME). To optimize conditions 3D-PCS-PDME), were manufactured a 3DX bioprinter. The scaffold-on-f...

We report on the vibronic structure of the ground state X̃(2)A″ of the thymine cation, which has been measured using a threshold photoelectron photoion coincidence technique and vacuum ultraviolet synchrotron radiation. The threshold photoelectron spectrum, recorded over ∼0.7 eV above the ionization potential (i.e., covering the whole ground state of the cation) shows rich vibrational structure ...